摘要
目的:探讨附子理中汤对脾阳虚证失眠大鼠下丘脑增食素(orexin)mRNA表达及下丘脑-垂体-肾上腺轴(HPAaxis)的影响及机制。方法:100只Wistar大鼠随机分为正常组、模型组、附子理中汤低剂量组,中剂量组和高剂量组5组,每组20只。模型组按400 mg.kg-1ip对氯苯丙氨酸(PCPA)每天1次,连续2 d,建立脾阳虚证失眠模型。低剂量组、中剂量组、高剂量组分别以附子理中汤10,20,40 g.kg-1ig,每日1次,连续7 d。疗程结束后对各组大鼠用ELISA法检测下丘脑5-羟色胺(5-HT)、促肾上腺皮质激素释放激素(CRH)及血浆促肾上腺皮质激素(ACTH)含量,RT-PCR法检测下丘脑orexin mRNA表达。结果:与正常组相比,模型组orexin mRNA表达量显著升高(P<0.01),5-HT含量显著降低(P<0.01);CRH,ACTH含量显著升高(P<0.05,P<0.01)。与模型组相比,附子理中汤高剂量组orexin mRNA表达量显著降低(P<0.01),附子理中汤高、中剂量组5-HT含量显著升高(P<0.01,P<0.05);高剂量组CRH,ACTH含量显著降低(P<0.05,P<0.01)。结论:附子理中汤下调orexin mRNA表达,抑制HPA轴激活,降低应激反应性,可能是其治疗失眠的机制之一。
Objective： To explore effect of Fuzi Lizhong decoction on the expression of hypothalamic orexin mRNA and hypcthalamopitutary-adrenol （HPA） axis in insomnia rats with spleen Yang deficiency syndrome and therapeutic mechanism. Method： Handred rats were randomly divided into control group, model group, low- dose group, middle-dose group, and high-dose group 5 groups, each group included 20 rats. Model rats were ip p- chlorophenylalanine （PCPA） according to 400 mg .kg-l, once a day for two days to establish insomnia model. low-dose group, middle-dose group, and high-dose group were ig given Fuzi Lizhong decoction respectively with 10, 20, 40 g ·kg-1 based on model group for 7 days. Result： Compared with control group, expression of orexin mRNA of model group increased significantly （P 〈 0. 01 ）. Compared with control group, content of 5-hydroxy tryptamine （5-HT） of model group reduced significantly （P 〈 0.01）. Compared with control group, content of cortieotropin-releasing bormone （CRH） of model group increased （P 〈 0.05）. Compared with control group, content of adrenocorticotropie hormone （ACTH） of model group increased significantly （P 〈 0.01 ）. Compared with model group, expression of orexin mRNA of treatment group reduced significantly （P 〈 0. 01 ）. Compared with model group, content of 5-HT of treatment group increased significantly （P 〈 0.01 ） and content of 5-HT in middle-dose group increased （P 〈 0. 05 ）. Compared with model group, content of CRH of treatment group reduced （P 〈 0.05 ）. Compared with model group, content of ACTH of treatment group reduced significantly （P 〈0.01 ）. Conclusion： Fuzi Lizhong decoction can reduce expression of Orexin mRNA and so that inhibit HPA axis to reduce stress reaction, which perhaps is one of mechanisms for insomnia treatment.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第16期289-292,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
附子理中汤
增食素
失眠
脾阳虚证
Fuzi Lizhong decoction
orexin
insomnia
spleen yang deficiency syndrome
