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一株高产二羟基丙酮菌株的筛选及鉴定 被引量:3

Isolation and indentification of a strain producing high 1,3-dihydroxyacetone
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摘要 从腐烂的水果中筛选到一株能发酵生产1,3-二羟基丙酮的菌株。基于生理生化鉴定及16S rRNA基因序列分析结果,确定该菌属于弗托氏葡萄糖酸杆菌,将其命名为Gluconobacter frateurii HD924。初步发酵实验表明该菌能发酵80 g/L的甘油产生63.04 g/L的1,3-二羟基丙酮。 A strain of Gluconobacter frateurii CGMCC 5397 producing 1,3-dihydroxyacetone from glycerol was isolated from rotted fruits. A series of morphological and biochemical characteristics and sequence analysis of 16S rRNA revealed that it belonged to Gluconobacter frateurii and named as Gluconobacter frateurii HD924. The fermentation results of this strain showed that 63.04 g/L 1,3-dihydroxyacetone could be produced when cultured in the medium containing 80 g/L glycerol.
出处 《工业微生物》 CAS CSCD 2013年第4期23-28,共6页 Industrial Microbiology
基金 河南省科技厅攻关项目(No.132102210387) 2011年度河南大学青年骨干教师培养计划资助
关键词 1 3-二羟基丙酮 甘油 弗托氏葡萄糖酸杆菌 鉴定 16S RRNA 1,3-dihydroxyacetone glycerol Gluconobacterfrateurii identification 16S rRNA
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  • 1李寅,曹竹安.微生物代谢工程:绘制细胞工厂的蓝图[J].化工学报,2004,55(10):1573-1580. 被引量:26
  • 2陈宏文,吴雅红,吴振华,方柏山,胡宗定.克雷伯杆菌甘油脱氢酶的分离纯化及性质[J].无锡轻工大学学报(食品与生物技术),2005,24(1):1-5. 被引量:11
  • 3冯屏,周家春,严希康,徐玉佩.弱氧化醋酸杆菌静止细胞氧化活性的研究及1,3-二羟基丙酮的制备[J].食品与发酵工业,2005,31(6):22-26. 被引量:8
  • 4陈可泉,韦萍,蔡婷,苏溧,龚璐,姜岷.反相高效液相色谱在发酵制备琥珀酸中的应用[J].生物加工过程,2005,3(2):50-52. 被引量:31
  • 5White SA, Claus GW. Effect of intracytoplasmic membrane development on oxidation of sorbitol and other polyols by Gluconobacter oxydans. J Bacteriol, 1982, 150 (2): 934-943.
  • 6Raska J, Skopal F, Komers K, et al. Kinetics of glycerol biotransformation to dihydroxyacetone by immobilized Gluconobacter oxydans and effect of reaction conditions. Collect Czechoslov Chem Commun, 2007, 72: 1269-1283.
  • 7Wei S, Song Q, Wei D, Production of Gluconobacter oxydans cells from low-cost culture medium for conversion of glycerol to dihydroxyacetone. Prep Biochem Biotechnol, 2007, 37(2): 113-121.
  • 8Hekmat D, Bauer R, Fricke J. Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans. Bioprocess Biosyst Eng, 2003, 26(2): 109-116.
  • 9Ohrem HL, Westmeier F. Microbial process for the preparation of dihydroxyacetone with recycling of biomass: US patent, 5770411. 1998-06-23.
  • 10Bauer R, Katsikis N, Varga S, et al. Study of the inhibitory effect of the product dihydroxyacetone on Gluconobacter oxydans in a semi-continuous two-stage repeated-fedbatch process. Bioprocess Biosyst Eng, 2005, 28(1):37-43.

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  • 1李峰,杨仲春.羟基乙酸的合成与应用[J].精细与专用化学品,2006,14(9):1-6. 被引量:13
  • 2田克胜,王保伟,许根慧.乙醇酸的合成及应用[J].天然气化工—C1化学与化工,2006,31(6):60-63. 被引量:23
  • 3刘宇鹏,郑璞,孙志浩.采用离子排斥色谱法分析发酵液中的琥珀酸等代谢产物[J].食品与发酵工业,2006,32(12):119-123. 被引量:42
  • 4郑裕国,胡忠策,柳志强,等.微生物法生产二羟基丙酮:CN,1821418[P],2006-08-23.
  • 5Mishra R, Jain SR, Kumar A. RETRACTED: Microbial production of dihydroxyacetone[J]. Biotechnology Advances, 2008, 26(4): 293-303.
  • 6Deppenmeier U, Hoffmeister M, Prust C. Biochemistry and biotechnological applications of Gluconobacter strains[J]. Applied Microbiology and Biotechnology, 2002, 60(3): 233-242.
  • 7Nabe K, Izuo N, Yamada S, et al. Conversion of glycerol to dihydroxyacetone by immobilized whole cells of Acetobacter xylinum[J]. Applied and Environmental Microbiology, 1979, 38(6): 1056-1060.
  • 8Asnis RE, Brodie AF. A glycerol dehydrogenase from Escherichia coli[J]. Journal of Biological Chemistry, 1953, 203(1): 153-159.
  • 9Streekstra H, de Mattos MJT, Neijssel OM, et al. Overflow metabolism during anaerobic growth of Klebsiella aerogenes NCTC 418 on glycerol and dihydroxyacetone in chemostat culture[J]. Archives of Microbiology, 1987, 147(3): 268-275.
  • 10Liu YP, Sun Y, Tan C, et al. Efficient production of dihydroxyacetone from biodiesel-derived crude glycerol by newly isolated Gluconobacter frateurii[J]. Bioresource Technology, 2013, 142:384-389.

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