摘要
目的:建立石墨炉原子吸收光谱法同时测定尿中铅(浓度变化范围大)的检测方法。方法:应用动态三磁场塞曼校正技术的石墨炉原子吸收光谱仪,通过磁场优化,采用氯化钯作基体改进剂,在283.3 nm波长下进行测定。结果:采用动态3-磁场模式测定时,还可同时得到2-磁场模式的数据和校正曲线,2-磁场标准曲线段(0.0μg/L^80.0μg/L):y=0.0048x+0.0145,r=0.9997;3-磁场标准曲线段(80.0μg/L^600.0μg/L):y=0.0013x+0.0308,r=0.9998;最低检出浓度2.0μg/L;回收率94.6%~104.8%;RSD为0.60%~3.72%。结论:该方法保持两种磁场模式的优点:检出限低、快速(样品无需再稀释)、准确、线性范围宽(0.0μg/L^600.0μg/L);可应用于职业接触和非职业接触尿中铅的同时测定,低浓度采用灵敏度较高的2-磁场模式数据处理,高浓度可采用线性范围宽的3-磁场模式计算。
Objective： To develop a method for direct determination of lead in urine by GF - AAS. Methods： Lead in urine samples was determined by GF - AAS with the dynamic thimagnetic field Zeeman correction technique at wavelength of 283.3 nm, with palladium chloride as the matrix modifier. Results： With dynamic trimagnetic field mode, data and the correction curve of two magnetic field can also be obtained, the standard curve （0 μg/L - 80 μg/L） was y =0. 0048x ＋0. 0145, r = 0. 9997 ; while the standard curve （ 80 μg/L - 600 μg/L） of 3 - magnetic field was y =0. 0013x ＋0. 0308, r =0. 9998. The detection limit was 2.0 μg/L; the recovery rate was 94.6% 104.8% ; RSD was 0.60% -3.72%. Conclusion： The method includes advantages of the two kinds of magnetic field models： low detection limit, rapidity （ sample without dilution again） , accuracy, wide linear range （0 μg/L - 600 μg/L）. The method can be used in the occupation exposure and non - occupation exposure of lead deter mination in urine. Low - concentration data was processed by the 2 - magnetic field standard curve, while high - con- centration data was processed by the 3 -magnetic field standard curve.
出处
《中国卫生检验杂志》
北大核心
2013年第7期1677-1679,共3页
Chinese Journal of Health Laboratory Technology
关键词
尿中铅
动态模式
三磁场塞曼
Lead in urine
Dynamic mode
Thimagnetic field- Zeeman effect background correction
