摘要
目的建立高效液相色谱(HPLC)法测定复方氯己定凝胶中醋酸氯己定和盐酸达克罗宁的含量。方法选用Inertsil ODS-SP色谱柱,0.5%三乙胺(用冰醋酸调pH值至3.8)-乙腈(68∶32)为流动相,流速1.0 mL/min,检测波长270 nm。结果醋酸氯己定在0.081 52～0.489 10μg时呈良好的线性关系(r=0.999 9,n=6),平均回收率为98.82%,相对标准偏差(RSD)为1.19%(n=9);盐酸达克罗宁在0.163 6～0.981 6μg时呈良好的线性关系(r=0.999 9,n=6),平均回收率为98.85%,RSD为1.13%(n=9)。结论 HPLC法操作简便、灵敏度高、重复性好,可用于该制剂中醋酸氯己定和盐酸达克罗宁的含量测定。
Objective To establish a HPLC method for determining the content of chlorhexidine acetate and dyclonine hydrochloride in Compound Chlorhexidine Gel.Methods The Inertsil ODS-SP chromatographic column was used with 0.5% triethylamine（adjusted pH to 3.8 with acetic acid）-acetonitrile（68∶32） as the mobile phase.The flow rate was 1.0 mL/min and detecting wavelength was 270 nm.Results The concentration of chlorhexidine acetate in the range of 0.08152-0.4891 μg showed a good linear relationship（r=0.999 9,n=6）,the average recovery rate was 98.82%,RSD 1.19%;the concentration of dyclonine hydrochloride in the range of 0.1636-0.9816 μg showed a good linear relationship（r=0.999 9,n=6）,the average recovery rate was 98.85%,RSD 1.13%（n=9）.Conclusion The HPLC method is simple to operate and highly sensitive with good repeatability,and can be used for the content determination of chlorhexidine acetate and dyclonine hydrochloride in this preparation.
出处
《现代医药卫生》
2013年第14期2108-2109,共2页
Journal of Modern Medicine & Health
关键词
复方
氯己定
凝胶类
苯丙酮类
色谱法
高压液相
Compounds（TCD）
Chlorhexidine
Gels
Propiophenones
Chromatography
high pressure liquid
