摘要
目的研究腺病毒介导的多基因（ｐ５３、Ｂ_（７－１）、ＧＭ—ＣＳＦ、ＩＬ－２）对肝癌细胞系凋亡的诱导，及对肝癌细胞系裸鼠体内成瘤性改变的影响。方法应用光镜、电镜和ＴＵＮＥＬ法，检测肝癌细胞系感染腺病毒介导的多基因后凋亡的发生。检测ＨｅｐＧ_２细胞导入腺病毒多基因后裸鼠体内的成瘤性改变。结果肝癌细胞系导入多基因后发生凋亡，并对化疗药物顺铂的敏感性增加，同时给予 １０ ｍｇ／Ｌ的顺铂，可使近 ３０％的肝癌细胞发生凋亡， 且裸鼠体内的成瘤性降低。结论腺病毒介导的多基因能诱导肝癌细胞发生凋亡，且与化疗药物顺铂协同，进一步诱导肝癌细胞发生凋亡。
Objective To investigate the apoptosis induced by adenovirus-mediated transduction of human genes (p53, B_(7-1), GM-GSF, IL-2) in liver cancer cells and the effects of tumorigenicity in nude mice. Methods Using light microscopy and electron microscopy and TUNEL assay, apoptosis in Ad - multigenes - transduced liver cancer cell lines was detected. The tumorigenicity of HepG_2 cells transduced with Ad-multigenes was detected. Results Ad-multigenes could induce apoptosis, and elevated sensitivity of human liver cancer cells to chemotherapy drugs. After cells with transduced Ad-multigenes were treated with 10 mg/L cisplatin for 24 h, apoptosis was seen in nearly 30% of them. The tumorigenicity of Ad-multigenes-transduced was reduced. Conclusion Apoptosis induced by adenovirus-mediated multigenes acts synergistically with cisplatin in promotion of inducing apoptosis in liver cancer cell lines.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第4期224-226,共3页
Chinese Journal of Hepatology
关键词
细胞凋亡
腺病毒
肝肿瘤
TUNEL法
Carcinoma, hepatocellular
Apoptosis
Recombinant adenovirus
Transduction, genetic
