摘要
目的研究表皮生长因子（ｅｎｉｄｅｒｍａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ， ＥＧＦ）、神经降压素（ｎｅｕｒｏｔｅｎｓｉｎ， ＮＴ）对四氯化碳损伤的原代培养肝细胞Ｃａ^（２＋）及膜流动性的影响。方法以Ｆｕｒａ－２／ＡＭ和ＤＰＨ荧光探针测定肝细胞内游离钙和膜流动性，观察ＥＧＦ、ＮＴ对四氯化碳损伤后肝细胞这两个指标的影响。结果ＥＧＦ、ＮＴ明显对抗肝细胞四氯化碳损伤后胞内［Ｃａ^（２＋）］ ｉ的增高和膜流动性的降低。结论 ＥＧＦ、 ＮＴ有维持肝细胞膜流动性和胞浆钙稳态的作用。
Objective To study the influence of epidermal growth factor (EGF), neurotensin (NT) on cytosolic calcium and membrane fluidity in carbon tetrachloride-injured primary cultured hepatocytes. Methods A micro-model of carbon tetrachloride -induced primary cultured hepatocytes injury was established. EGF and NT were added to the cells 1 h ahead of carbon tetrachloride given. Twenty-four hours later, cytosolic calcium and membrane fluidity in hepatocytes were detected with fluorescence probe of Fura-2/AM and 1, 6-dihenyl-1, 3, 5-hexatriene (DPH). Results EGF and NT could significantly antagonize the increase of cytosolic Ca^(2+) and decrease of membrane fluidity in carbon tetrachloride-injured hepatocytes. Conclusion EGF and NT have a function of maintaining membrane fluidity and steady-status of cytosolic calcium of hepatocytes.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第4期218-220,共3页
Chinese Journal of Hepatology
基金
福建省自然科学基金!(C95055)
关键词
四氯化碳中毒
膜流动性
肝细胞
钙离子
EGF
NT
Epidermal growth factor
Carbon tetrachloride poisoning
Membrane fluidity
Hepatocytes
