摘要
目的 观察谷氨酰胺强化的TPN和生长激素对异基因小肠移植受体大鼠蛋白质代谢的调节作用。 方法 2 4只异基因异位小肠移植受体大鼠 (SD→Wistar) ,根据术后营养支持方案的不同随机分为 4组 ,每组 6只大鼠 :Ⅰ组 :标准TPN组 (standardTPN ,STPN) ;Ⅱ组 :谷氨酰胺强化TPN组 (glutamine enrichedTPN ,GTPN) ;Ⅲ组 :重组人生长激素 (rhGH)加STPN组 (GH/STPN) ;Ⅳ组 :rhGH加GTPN组 (GH/GTPN)。所有大鼠均应用环孢素A抑制排斥反应。观察移植后即刻至 14d内受体大鼠体重、氮平衡、尿 3 甲基组氨酸排泄量和血浆白蛋白浓度的变化。 结果 谷氨酰胺强化的TPN及生长激素能够减轻小肠移植术后受体大鼠的蛋白质分解 ,促进蛋白质合成和正氮平衡 ,减少体重下降幅度。 结论 谷氨酰胺和生长激素能够有效地改善小肠移植术后机体的蛋白质代谢 ,有助于受体整体情况的改善 ,二者联合应用具有协同作用。
Objective[WT5”BZ] To observe the effect of glutamine enriched TPN and recombinant human growth hormone (rhGH)on protein metabolism of recipient of rat small bowel transplantation. [WT5”HZ]Methods[WT5”BZ] Twenty four recipients of rat allogeneic heterotopic small bowel transplantation(SD→Wistar rat) were devided into 4 groups randomly according to the presence or absence of glutamine or rhGH in TPN regimen. Cyclosporine A(CsA) was used as the immunosuppressant. The changes of body weight, nitrogen balance, urinary 3 methyl histidine excretion and plasma albumine level during 14 days after transplantation were observed. [WT5”HZ]Results[WT5”BZ] Applications of glutamine enriched TPN or rhGH decreased the postoperative catabolism of protein of recipients. They promoted protein synthesis and positive nitrogen balance so as to minimize the loss of body weight and correct hypoalbuminemia. They were synergistic to other. [WT5”HZ]Conclusions[WT5”BZ] Glutamine entiched TPN and rhGH can promote the protein metabolism of rat small bowel transplantation recipients effectively with the use of CsA, so as to improve the recovery of general condition. Their combination may have the synergistic effect. [WT5”HZ]
出处
《中华外科杂志》
CAS
CSCD
北大核心
2000年第8期622-624,共3页
Chinese Journal of Surgery
关键词
谷氨酰胺
生长激素
小肠移植受体
蛋白质代谢
Transplantation, homologous
Glutamine
Somatropin
Metabolism
Rats,inbred BB
