摘要
目的研究中链甘油三酯对72h连续睡眠剥夺大鼠的认知能力和抗氧化能力的影响。方法将30只雄性SD大鼠随机分为正常睡眠组(NC组)、睡眠剥夺组(SD组)、低剂量MCT组(LD组)、中剂量MCT组(MD组)和高剂量MCT组(HD组),用不含MCT的饲料喂养NC组和SD组,用含低、中、高剂量中链甘油三酯的饲料喂养LD组、MD组、HD组。4周后,利用睡眠剥夺箱对大鼠进行睡眠剥夺连续72h后,使用Y型电迷宫来检测大鼠的认知能力,并检测大鼠肝脏中丙二醛(MDA)含量以及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性。结果 72h连续睡眠剥夺后,SD组大鼠的迷宫反应正确率显著低于其余各组,SD组与NC组相比,MDA含量增加(P<0.05),GSH-Px和SOD活力下降(P<0.05,P<0.01);LD组和MD组的MDA均显著低于SD组(P<0.05,P<0.01),MD组的GSH-Px活性显著高于SD组(P<0.05)。MCT对SOD的活性没有显著作用。结论 72h睡眠剥夺使大鼠的认知能力和抗氧化能力明显下降,而中链甘油三酯能改善睡眠剥夺大鼠的认知能力和抗氧化能力。
Objective To investigate the effect of medium - chain triglycerides （MCT） on the cognitive ability and antioxidant ca- pacity of rats with continuous 72 - hour sleep deprivation （SD）. Methods Thirty rats were randomly divided into the normal control group （NC group ）, blank sleep deprivation group （SD group）, low -dose MCT group （LD group）, medium -close MCT group （MD group ） and high - dose MCT group （ HD group）. Sleep deprivation tank was used to deprive the sleep of rats. After continuous 72h, a Y - shaped electric maze was used to measure the learning and memory abilities of rats. Then, the level of GSH - Px, MDA and SOD in rat liver was detected. Results The rats in experimental group had a significantly lower correct response rate than the control group in Y - shaped electric maze （P 〈 0.05）. The activity of GSH - Px and SOD in SD group was significantly lower than NC group （ P 〈 0.05,P 〈 0.01 ）. The content of MDA in SD group was significantly higher than NC group. The content of MDA in LD/MD group was significantly lower than SD group. The activity of GSH - Px in MD group was significantly higher than SD group. Conclusion Continuous sleep depri- vation can decrease the cognitive ability and antioxidant capacity of rats significantly. MCT can protect the the cognitive ability and antioxi- dant capacity of rats after sleep deprivation.
出处
《医学研究杂志》
2013年第8期70-73,共4页
Journal of Medical Research
基金
上海市体育局基金资助项目(04JT017)
关键词
中链甘油三酯
大鼠
认知能力
抗氧化能力
Medium -chain triglycerides （MCT）
Rats
Cognitive ability
Antioxidant capacity
