摘要
目的 通过检测强直性脊柱炎患者外周血中CD4+ 和CD8+ T淋巴细胞分泌细胞因子的情况 ,探讨T淋巴细胞亚群与强直性脊柱炎发病的关系。方法 建立三色流式细胞分析法对强直性脊柱炎患者和健康志愿者外周血中淋巴细胞的胞内细胞因子 (IFNγ、IL 4)和表面抗原 (CD4、CD8)进行分析。同时采用夹心酶联免疫吸附法测定血清中IFNγ和IL 4的水平。结果 无论是CD4+ 还是CD8+ T淋巴细胞 ,分泌IFNγ的细胞群要比分泌IL 4的细胞群多 ;IL 4主要由Th2细胞产生 ,而Th1分泌的IFNγ要比Th2多 ;患者的Th1或Th2型细胞数与炎症活动指标呈负相关。与对照组相比 ,强直性脊柱炎患者外周血中Th1和Th2细胞在受刺激后并未增多。患者外周血中IFNγ水平明显升高 ,而IL 4水平无显著变化 ;IFNγ水平与炎症活动指标无明显相关性。结论 在强直性脊柱炎患者的外周血淋巴细胞中 ,以Th1型细胞为主。但Th1细胞在受刺激后分泌细胞因子的能力比Th2细胞低。
Objective To investigate the pattern of T helper cytokines in the peripheral blood and try to understand the role of T lymphocyte subgroups in the pathogenesis of ankylosing sondylitis (AS).Methods Three color flow cytometric analysis was employed for intracellular cytokines (IFNγ,IL 4) and surface antigens (CD4,CD8) of T cells in the peripheral blood of AS patients and healthy volunteers.Sandwich ELISA was used to measure the level of IFNγ and IL 4 in the serum.Results The positive rates of IFNγ producing cells among either CD4 + or CD8 + T cells were significantly higher than those of IL 4 producing cells in the peripheral blood of patients with AS.Although the rates of IFNγ producing cells among CD4 + and CD8 + T cells in AS patients did not change compared with the controls,they had a negative correlation with the inflammation index (CRP,ESR and platelet counts).The level of IFNγ was increased while the level of IL 4 was within the normal range,and the IFNγ/IL 4 ratio in the patients was significantly higher than that in the controls.But neither the level of IFNγ nor the IFNγ/IL 4 ratio had any relation with the inflammation index.Conclusion There is a dominant Th1 subset in the peripheral blood of the AS patients,that is,there is a shift toward T cells with a Th1 cytokine profile.But Th1 cells seem to secrete fewer cytokines after stimulated by PMA/ionomycin than Th2 cells.
基金
国家自然科学基金资助项目!(A3 9870 72 0 )
