摘要
本试验以样品加标回收率为指标，比较了液一液萃取、直接提取、亲和层析三种前处理方法对饲草中黄曲霉毒素B1测定值准确性的影响。应用酶联免疫法（ELISA）进行测定，并通过液相色谱荧光检测法（HPLC）进行验证。结果，采用液-液萃取-ELISA法，平均回收率在70．0％～102．0％，且相对标准偏差（RSD）〈6．6％，亲和层析净化后ELIsA与HPLC结果基本一致，但较液-液萃取测定值低。提示液一液萃取法作为ELISA检测饲草中黄曲霉毒素B1的前处理方法为最佳方法。
To determine the best pretreatment of aflatoxin B1 in forage by ELISA, this paper compared liquid-liquid extraction, direct extraction and immunoaffinity chromatography with standard recovery rate Of samples, and then verified by HPLC with fluorescence detection. As for liquid-liquid extraction, the average recovery rate is 70.0%-102.0%, and relative standard deviation（RSD） is less than 6.6%. ELISA is roughly identical to the rusults of HPLC, but it is lower than liquid-liquid extraction. The result showed that, it is feasible and accurate to determine AFB~ by liquid-liquid extraction pretreatment method in forage.
出处
《中国奶牛》
2013年第11期12-15,共4页
China Dairy Cattle
