摘要
目的分析永州市2012年1-12月手足口病(HFMD)的病原学特征,为手足口病的预防控制工作提供病原学依据。方法选取2012年1-12月辖区内三级甲等综合性医院定点收治的278例手足口病病例作为研究对象。采集HFMD患儿发病1周内的咽拭子标本,用实时荧光RT-PCR法对患者进行总肠道病毒、柯萨奇病毒A组16型(CoxA16)和肠道病毒71型(EV71)的特异性核酸检测。回顾性分析患儿性别、年龄、病例来源的分布情况。结果 278例手足口病病例中,肠道病毒通用型核酸阳性182份,检出率65.47%(182/278),其中EV71、CoxA16、EV71和CoxA16混合感染、非EV71和CoxA16的其他肠道病毒核酸分别为151、1、1、29份,占肠道病毒感染阳性的82.97%(151/182)、0.55%(1/182)、0.55%(1/182)、15.93%(29/182),非肠道病毒(NEV)96例占34.53%(96/278)。患儿以3岁以下儿童为主,男性多于女性,以散居儿童居多。结论 2012年1-12月永州市HFMD流行的病原体优势株可能为EV71,实时荧光RT-PCR可用于手足口病的快速诊断,对3岁以下儿童手足口病进行病原学检测,及早发现及早治疗,是防止手足口病死亡病例的关键。
Objective To analyze the aetiological characteristics of hand,foot and mouth disease（HFMD） in Yongzhou from January to December 2012,and to provide aetiological evidence for prevention and control of HFMD.Methods Totally,278 cases of HFMD hospitalized in a grade A class 3 hospital from January to September 2012 were selected to serve as research subjects.Throat swab specimens of children with HFMD were collected within 1 week of symptom onset.Real-time fluorescence RT-PCR method was used to detect specific nucleic acids of general enterovirus,Coxsackie virus group A type 16（CoxA16） and enterovirus 71（EV71）.The distribution of patients＇ sex,age,and case sources was retrospectively analyzed.Results Totally,182（65.47%） of 278 HFMD cases were positive with enterovirus universal nucleic acid;among them,EV71,CoxA16,EV71 and CoxA16 mixed infection,non-EV71 and CoxA16 other intestinal virus which were positive with nucleic acids accounted for 82.97%（151/182）,0.55%（1/182）,0.55%（1/182）,and 15.93%（29/182）,respectively.Non-enterovirus（NEV） infection accounted for 34.53%（96/278）.Most of the patients were scattered children under 3 years of age,with more males than females.Conclusions EV71 may be the predominant circulating strain causing HFMD in Yongzhou from January to December 2012.Real-time fluorescence RT-PCR can be used for rapid diagnosis of hand,foot and mouth disease.Pathogen detection for HFMD among children under 3-year-old,early detection and early treatment are the key points to prevent HFMD deaths.
出处
《实用预防医学》
CAS
2013年第7期879-881,共3页
Practical Preventive Medicine
