摘要
用薄层色谱法分离测定了反应混合物中的 1 ,5-苯并硫氮杂 艹卓-α-氨基 -β-内酰胺 .扫描条件为 :λ1 =2 80 nm,λ2 =3 54nm,狭缝 1 .2 mm× 1 .2 mm,SX=3 .校准曲线的线性范围是 0 .2 75～ 7.70 μg( γ=0 .990 5,n=9) ,最低检出限为 0 .1 4 0μg.方法的变异系数小于 5% ,平均回收率为 1 0 0 .1 5% ,方法简便。
A method for separation and determination of 1,5 benzothiazepines α amino β lactam in reaction mixture by thin layer chromatography has been established.The operating conditions are: λ 1=280nm, λ 2=354nm,beam size 1.2mm×1.2mm, SX =3.The calibration curves are linear in the range of 0.275～7.70μg( γ =0.9905, n =9),the detectable limit is 0.140μg. The coefficient of variation of this method is less than 5%,the average recovery for β lactam is 100.15%.The method is simple and rapid.
出处
《河北师范大学学报（自然科学版）》
CAS
2000年第3期351-352,共2页
Journal of Hebei Normal University：Natural Science
基金
河北省教委资助课题!( 5 83 0 8)
