摘要
目的:用血清药理学方法研究鹅不食草挥发油对抗原诱导RBL-2H3细胞释放组胺、β-氨基已糖苷酶,对刀豆蛋白(Con-A)诱导小鼠脾细胞增殖、诱导脾淋巴细胞分泌IL-4的影响,探讨其抗变态反应的可能机制。方法:ELISA法测定鹅不食草挥发油含药血清对抗原诱导RBL-2H3释放组胺、β-氨基已糖苷酶的影响;MTT法测定鹅不食草挥发油含药血清对Con-A诱导脾细胞增殖的影响,ELISA法测定鹅不食草挥发油含药血清对Con-A诱导脾淋巴细胞分泌IL-4的影响。结果:与空白血清相比,鹅不食草挥发油0.5～2h含药血清(9g/kg生药量,10%含药血清)均具有抑制抗原诱导RBL-2H3释放组胺、β-氨基已糖苷酶,抑制Con-A诱导小鼠脾细胞增殖、分泌IL-4的作用。结论:鹅不食草挥发油抗变态反应作用机制可能与抑制炎症介质释放,抑制细胞免疫功能有关。
Objective：Use serum pharmacological method to study the effect of volatile oil of Small Centipeda Herb on release of histamine and β-hexosaminidase in RBL-2H3,on the proliferation of splenocytes and secretion of IL-4 induced by Con-A.Investigate the treatment mechanism of volatile oil of Small Centipeda Herb in allergic reaction.Method：Histamine,β-hexosaminidase released from RBL-2H3 infulenced by drug-containing serum sampled after taken volatile oil of Small Centipeda Herb were measured by ELISA.MTT assay was used to measure the inhabitation of proliferation of splenocytes induced by Con-A but influenced by drug-containing serum sampled after taken volatile oil of Small Centipeda Herb.Measured IL-4 secreted from spleen lymphocytes which induced by Con-A but influenced by drug-containing serum sampled after taken volatile oil of Small Centipeda Herb by ELISA.Result：Compared with blank serum,drug-containing serum（9g/kg,10% serum concentration）can reduce the total release of histamine and β-hexosaminidase from RBL-2H3 cells induced by antigen,inhibit proliferation of splenocytes and IL-4 secretion induced by Con-A,the differences were significant（P0.05）.Conclusion：The treatment mechanism of volatile oil of Small Centipeda Herb at allergic reaction may be associated with the inhibition of the release of inflammatory mediators and the inhibition of cellular immune function.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第1期78-80,共3页
Pharmacology and Clinics of Chinese Materia Medica
关键词
鹅不食草
挥发油
抗炎
免疫抑制
血清药理学
Small Centipeda Herb（鹅不食草）
volatile oil
anti-inflammatory
immuno-suppression
serum pharmacological
