摘要
目的:探讨阿魏酸钠对糖尿病大鼠肾脏的保护机制。方法:50只雄性SD大鼠随机分为2组,正常对照组10只,造模组40只。造模组采用空腹一次性尾静脉注射链脲佐菌素(STZ)55mg/kg制造糖尿病模型,72小时后血糖≥16.7mmol/l为造模成功。将造模成功的40只大鼠随机分为糖尿病模型组、阿魏酸钠50mg/kg、100mg/kg、200mg/kg,给药8周后全自动化生化分析仪检测生化指标,免疫组化法和Western Blot法检测转化生长因子-β1(TGF-β1)、Smad1、Ⅳ型胶原(ColⅣ)定位和半定量表达,电镜观察肾脏组织超微结构。结果:与糖尿病模型组相比,阿魏酸钠高中低剂量组基底膜增厚、系膜基质积聚、足突融合均减轻,肾脏组织中TGF-β1、Smad1、ColⅣ表达减少,且有剂量依赖性,高剂量组改变最明显。结论:阿魏酸钠对糖尿病肾脏具有保护作用,可能与降低TGF-β1、Smad1、ColⅣ的表达有关。
Objiective： To study the protection mechanism of sodium ferulate（SF） on diabetic rats＇ kidney.Methods ： 50 male SD rats were randomly allocated into 2 groups： normal control group（n = 10）,modle group（n = 40）.Rats in the modle group were administered with strepto-zotocin（55mg/kg） by vena caudalis injection to induce diabetic model.The modle diabetes mellitus were considered to be successful when the concentration of blood glucose was constantly ≥16.7mmol/l 72 hours after the injection.The modle rats were divided randomly into 4 groups： diabetes mellitus modle group（n = 10）,SF high dosages treatment group（n = 10）,SF middle dosages treatment group（n = 10） and SF low dosages treatment group（n = 10）.The latter 3 groups were given corresponding dosages of 50mg /（kg.d）,100mg /（kg.d） and 200mg /（kg.d）.The interference lasted for 8 weeks.Biochemical indices were obtained by automatic analyzer.Immunohistochemical method and Western Blotting analysis were used to detect the protein expression of smad1 and transforming growth factor-β1（TGF-β1）,type Ⅳcollagen（Col Ⅳ）.The Renal ultrastructure was observed by electron microscope.Results： Comparing with diabetes modle group,SF high,middle and low groups can reduce the expansion of mesangial matrix and thickening of the glomerular basement membrane,downgrade the expression of Smad1,TGF-β1 and ColⅣwith dosages-response relationship.SF high group changes more obvious.Conclusion： SF on diabetic kidney may have a protective effect.The mechanism may relate with reducing the expressing of TGF-β1,Smad1 andColⅣ.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第1期57-60,共4页
Pharmacology and Clinics of Chinese Materia Medica
