摘要
目的:探讨人参皂苷Rg1对腹主动脉缩窄(AAC)致心肌肥厚的抑制作用及其机制。方法:大鼠采用腹主动脉结扎的方法制备AAC模型,1周后ig给予大鼠人参皂苷Rg1 4,2,1 mg/kg,每天1次,共11周。计算心脏质量指数(HMI)和左心室质量指数(LVMI);测定左心室收缩压(LVSP)、左心室舒张末压(LVEDP)和左心室内压最大升降速率(±dp/dt max),HE染色观察大鼠左室心肌形态学改变;采用逆转录-聚合酶链反应(RT-PCR)测定心肌ANP和TLR4 mRNA表达;采用Western blot测定p65和IκBα的蛋白表达,采用ELISA测定血清中TNF-α和IL-1β的表达。结果:与假手术组相比,AAC模型组的大鼠IκBα明显降低,其他指标均显著增高。与模型组相比,人参皂苷Rg1 4 mg/kg组HMI,LVMI,LVSP,LVEDP和±dp/dt max均显著降低(P<0.05);人参皂苷Rg1 4,2 mg/kg组ANP mRNA,TLR4 mRNA,p65蛋白表达,血清中TNF-α和IL-1β表达明显下降(P<0.05),IκBα蛋白表达明显升高(P<0.05)。结论:人参皂苷Rg1能够有效抑制AAC大鼠心肌肥厚并改善炎症反应,其机制可能与抑制TLR4/NF-κB信号通路表达有关。
Objective： To explore the effect of Ginsenoside Rg1 on the hypertrophic myocardium caused by abdominal aorta constriction（AAC）in rats,and to explore its molecular mechanism.Methods： Myocardial hypertrophy model in rats was established by adopting AAC.Ginsenoside Rg1 4,2 and 1 mg/kg were ig given to the rats for 11 weeks.Heart mass index（HMI） and left ventricular mass index（LVMI） were detected.Left ventricular systolic pressure（LVSP）,left ventricular end-diastolic pressure（LVEDP）,and maximum rate of rise/decrease of LVSP（±dp/dtmax） were detected,and pathological section by HE staining.The expression of ANP and TLR4 mRNA were determined by RT-PCR.The expression of p65 and IκBα were assessed by Western blot.TNF-α and IL-1β were measured by ELISA.Results： Compared with sham group,IκBα significantly decreased and other indicators significantly elevated in all index in AAC group.Compared with AAC group,Ginsenoside Rg1 4 mg/kg decreased HMI,LVMI,LVSP,LVEDP and ±dp/dtmax（P0.05）.Ginsenoside Rg1 4 and 2 mg/kg could also remarkably down-regulate the over expression of ANP and TLR4 mRNA and p65 protein and TNF-α and IL-1β and significantly improved IκBα protein expression（P0.05）.Conclusion： Ginsenoside Rg1 significantly ameliorate myocardial hypertrophy and inflammatory in AAC rats and the mechanism may be related to the inhibition of TLR4/NF-κB.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第1期35-38,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
辽宁省自然科学基金(201102141)
