摘要
背景:SOD1-G93A转基因小鼠是研究肌萎缩侧索硬化的经典代表性疾病模型动物,然而对于这种转基因小鼠在保种过程中所存在的种系退化现象却少有报道。目的:研究家族性肌萎缩侧索硬化小鼠在保种过程中出现的种系退化现象。方法:通过比较家族性肌萎缩侧索硬化疾病模型SOD1-G93A转基因小鼠在保种过程中不同代间的怀孕胎数、产仔数以及子代鼠中雄性和阳性小鼠所占得比例分析该转基因小鼠保种过程中生育能力的变化,并比较不同代间小鼠的起病时间、生存期以分析该转基因小鼠在保种过程中发病情况的变化。结果与结论:第3,6代小鼠与第0代比较,其怀孕胎数、每胎产仔数、每只产仔数等均显著下降(P<0.05),且第6代小鼠中有一半以上雌鼠无法受孕。其子代鼠中雄性小鼠以及阳性小鼠所占的比例有下降趋势,但是其差异无显著性意义。第6代小鼠与第1代比较其发病时间与死亡时间分别推迟了6.37d(P=0.004)和9.67d(P=0.022),差异有显著性意义。以上结果显示家族性肌萎缩侧索硬化小鼠在保种过程中存在生育能力下降、发病及死亡时间推迟等种系退化现象。
BACKGROUND： SOD1-G93A transgenic mouse is a classical morbid animal model in the investigation of amyotrophic lateral sclerosis. However, there are few reports about the germline deterioration of this transgenic mouse model when breeding.OBJECTIVE： To investigate the germline deterioration of mouse model of familial amyotrophic lateral sclerosis when breeding. METHODS： The times of pregnancy, litter size among different generations and the ratios of male and positive of the filial generation were comPared to analyze the change of fertility of amyotrophic lateral sclerosis mouse model of SOD1-G93A transgenic mouse when breeding. And the times of invasion and death were compared to analyze the change of pathogenesis of this transgenic mouse when breeding. RESULTS AND CONCLUSION： The times of pregnancy, per litter size and per mice were decreased significantly （P 〈 0.05） when comparing the third and sixth generation with the first generation, and moreover there were over half of the mouse of the sixth generation who can not inbreed. The ratios of male and positive mouse of the filial generation also decreased, but the difference was not significant. The time of invasion and death delayed significantly by 6.37 days （P=-0.004） and 9.67 days （P=0.022） respectively when comparing the sixth generation with the first generation, and the difference was significant. As a result, familial amyotrophic lateral sclerosis mouse showed deteriorated germline when breeding such as the fertility decline and the delay of the time of invasion and death.
出处
《中国组织工程研究》
CAS
CSCD
2013年第24期4521-4528,共8页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81030019
30871359)
北京市自然科学基金(7082099
7102161)
教育部博士点基金(20100001110084)
首都医学科技发展基金(SF-2009-III-34)~~
关键词
组织构建
组织构建基础实验
肌萎缩侧索硬化
转基因小鼠
疾病动物模型
种系退化
繁殖
国家
自然科学基金
tissue construction
basic experiments in tissue construction
amyotrophic lateral sclerosis
transgenic mouse
morbid animal madeligermline deterioration
breeding
National Natural Science Foundationof China
