摘要
目的:研究榄香烯对胃癌耐药细胞株SGC7901/ADM耐药性的影响及机制。方法:耐药肿瘤细胞SGC7901/ADM分为对照组和榄香烯处理组,MTT法检测各组对胃癌细胞株的细胞毒作用,免疫细胞化学染色法检测各组胃癌细胞株中的核转录因子活化,流式细胞仪检测胃癌细胞株凋亡率。结果:免疫细胞化学染色显示SGC7901/ADM胃癌细胞于阿霉素处理后9～12 h后可检测到核转录因子活化,最高达到60～65%,而榄香烯处理组胃癌细胞株可检测到核转录因子活化明显降低,并随其浓度升高呈反比,最低至8～12%。SGC7901胃癌细胞株凋亡率随榄香烯浓度而升高(5.30±0.81)%～(48.53±1.02%),在同一浓度下,凋亡率随ADM作用时间的延长而升高。MTT法结果示非细胞毒浓度的榄香烯能增强ADM对SGC7901/ADM细胞的杀伤作用,并与ADM有协同作用,作用强度与榄香烯剂量呈正相关。结论:榄香烯可逆转胃癌细胞株耐药性,抑制核因子-κB活化可能在其中发挥一定作用。
Objective ： To investigate the effect of elemene on reversing chemoresistance to adriamycin （ADM） in human stomach cancer cell, and explore its possible mechanism. Methods：SGC7901/ADM were divided imto two groups： control group and elemene treatment group. The cytotoxieity of ADM on SGC7901/ADM was determined by MTT assay. The activity of nuclear factor-kappa B （NF-KB） was measured by immunohistochemieal staining. The apoptosis rate of stomach cancer cell line was determined by flow cyotometric analysis. Results ：The immunohistoehemieal staining result showed that the activity of NF-KB in SGC7901/ADM was increased after treated with ADM for 9 - 12 h, while that of the elemene treatment group decreased with the increasing of the elemene concentra- tion and the lowest level was 8 - 12%. The apoptosis rate of SGC7901/ADM stomach cancer cell line was increased with the incre.asing of elemene concentration. At the same elemene concentration, the apoptosis rate increased with ADM treatment time prolonged. MTT result showed that the non-eytotoxie dose of elemene had synergistic effect on rilling SGC7901/ADM stomach cancer cell line and was in a dose-dependant manner. Conclusion ： The inhibitory effect of elemene on reversing chemoresistance to ADM in human stomach cancer cell line maybe related to inhibiting NF-KB activity.
出处
《中药材》
CAS
CSCD
北大核心
2013年第4期601-603,共3页
Journal of Chinese Medicinal Materials
关键词
榄香烯
阿霉素
核因子-ΚB
耐药性
胃癌细胞株
凋亡
Elemene
Adriamycin
Nuclear factor-kappa B
Chemoresistance
Stomach cancer cell line
Apoptosis
