摘要
MicroRNA(miRNA)是一类内源性的、长度约18～24 nt、非编码、单链小RNA分子,miRNA广泛存在于各种动植物体内,并参与基因表达的调控。随着植物miRNA研究的不断发展,越来越多的miRNA被证实在不同的非生物逆境下具有重要的调控作用,例如营养胁迫、低温胁迫、机械胁迫等。该文综述了植物miRNA的合成、miRNA及其靶基因的鉴定方法,并重点介绍了非生物逆境相关miRNA的表达分析方法、逆境条件下miRNA的调控特征及其靶基因特点等。
MicroRNAs（miRNAs） are a class of endogenous,small（～18-24 nt）,single-stranded,non-coding RNAs that widely distribute in animals and plants and participate in the regulation of gene expression.With the development of the research of plant miRNA,more and more miRNAs was convinced to play important regulatory roles in the condition of abiotic stresses,e.g.,nutrition starvation,low temperature,mechanism damage and so on.This paper reviews the synthesis of miRNA,the identification of miRNA and the targeted gene,and introduce the express analysis of stress-related miRNA,the regulatory pattern of miRNA and the property of the targeted gene.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2013年第4期673-677,共5页
Journal of Anhui Agricultural University
基金
中国博士后科学基金项目(2013M531498)
国家自然科学基金(31171608)共同资助
