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利用线粒体COⅠ序列快速检测狐源性成分 被引量:5

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摘要 狐狸是一种重要的经济皮毛动物,养殖业十分发达。选择狐狸线粒体COⅠ基因序列,设计特异性引物和探针,经条件优化和验证,建立狐狸成分的实时荧光PCR检测方法。该方法灵敏度高,检测限可达12 fg DNA,而且与常见的猪、牛、羊、犬以及大豆、玉米等其他物种无交叉反应。该方法可作为食品和饲料中狐狸源性成分鉴别检测的有效方法,也可作为狐狸皮毛真伪鉴别的方法。
出处 《畜牧与兽医》 北大核心 2013年第7期57-60,共4页 Animal Husbandry & Veterinary Medicine
基金 国家质检总局项目(2011IK011)
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同被引文献110

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