摘要
研究了不同金属离子对纤维素酶活的影响。重点阐述了从R2工程菌分离纯化纤维素酶的方法。结果表明:Cu2+对酶活有一定的抑制作用,其余各种离子在一定浓度范围内对酶活都有促进作用;在Ca2+、Cu2+、Zn2+的实验中,Ca2+促进作用大于Zn2+;在K+、Na+、Li+的实验中,Li+促进作用明显;对于不同配比的K+、Na+、Li+的实验中,K+∶Na+∶Li+(1∶1∶2)的促进作用较明显。表明在反应中加入一定的金属离子对纤维素酶活有一定的促进作用。
The effects of different metal ions on cellulase were studied.This research focused on the method of separating and purification cellulase from R2 engineering strains.The results are as follow ： cellulase activity decreased by Cu2 ＋,while the others could improve the cellulase activity within a certain range of concentration;among Ca2 ＋,Cu2 ＋and Zn2 ＋,the activation effect of Ca2 ＋ is stronger than that of the Zn2 ＋;among K＋,Na＋and Li＋,Li＋ had an obvious effect;in the test of K＋,Na＋ and Li＋ with different ratio,K＋： Na＋： Li＋（1： 1： 2） had a relatively obvious effect,which showed that the addition of certain mental ions had an activation effect on the cellulase.
出处
《科学技术与工程》
北大核心
2013年第18期5395-5398,共4页
Science Technology and Engineering
基金
重庆高校优秀成果转化项目(kjzh11216)
重庆市教委科学技术研究项目(KJ120811)资助
