摘要
目的:比较微柱凝胶法和传统试管法检测O型孕妇血型抗体效价的差异,探讨微柱凝胶试剂卡检测孕妇IgG效价的界值。方法:用微柱凝胶试剂卡和试管抗人球蛋白平行检测O型血孕妇血清IgG抗体效价。结果:微柱凝胶试剂卡比试管法检测数高2个稀释度,微柱凝胶试剂以1∶256为界值与试管法比较,阳性检出率差异无统计学意义(P>0.05),结果一致性较好(χ2=191.340,P<0.01,Kappa指数=0.542)。结论:两种试剂检测的一致性好,使用微柱凝胶卡比试管法操作更简单,结果更易判读,值得临床推广。本研究推荐其临床参考值应设为≥1∶256。
Objective ： To explore the micro - column gel Coombs test and test tube Coombs test for IgG titre detection in serum of pregnant women with blood type O, and to establish the critical titre for IgG micro - column gel Coombs test. Methods ： Blood samples of pregnant women with blood type O were detected simultaneously by the micro - column test and the tube test. Results ： On average, two titers higher was found with the micro - column gel Coombs test than with the test tube Coombs test. No statistically significant difference in the positive rate was found between the two methods with 1：256 of micro -column gel reagent as the field value （P 〉0.05）. The two methods had a high correlation （X2 = 191. 340,P 〈0. 01,Kappa index： 0. 542）. Conclusion： The two detection reagents are in good consistency. The method of micro - column gel Coombs test is simpler, more standardized and its result is easier to judge, resulting in more worthy of clinical use. In addition, the clinical reference value suggested by this study should be not less than 1：256.
出处
《中国计划生育学杂志》
2013年第7期464-465,471,共3页
Chinese Journal of Family Planning
