摘要
目的对抗-HCV反应性献血者进行随访以分析反应性献血者的归队途径。方法随机对上海地区52名抗-HCV单试剂反应性献血者献血间隔6个月后随访,使用4种采供血机构常用的抗-HCV ELISA试剂检测,对于抗-HCV反应性标本使用重组免疫印迹试验确证,同时使用罗氏cobas Taqscreen MPX核酸检测试剂(NAT)单人份检测;3～6个月后进行第2次随访,开展相同实验。结果 2次随访研究发现,52名献血者中,39名(75%)献血者4种抗-HCV试剂检测结果均为阴性,13名(25%)献血者4种抗-HCV试剂至少有1种试剂检测结果为反应性,与原抗-HCV试剂检测结果相同,且S/CO数值保持基本一致,13名抗-HCV反应性献血者中有1名免疫印迹检测结果为阳性;52名献血者NAT结果均为阴性。结论献血者抗-HCV单试剂反应性,经过至少6个月间隔,使用包含原抗-HCV在内的2种试剂检测结果阴性,同时NAT检测阴性者可恢复献血权利。
Objective To conduct a follow-up study on the blood donors with reactive anti-HCV results, and to provide pilot study data for drawing a donor reentry route in China. Methods Randomly selected 52 blood donors with an- ti-HCV reactive results （1 of 2 ELISA screening assays）, were called back at least 6 months after the last donation. They were tested for the presence of anti-HCV with 4 different commonly used anti-HCV ELISA assays in blood transfusion ser vices. The sample, which was reactive with ELISA, was confirmed for the presence of anti-HCV with WB and tested for the HCV RNA with Roche Cobas Taqscreen MPX nucleic acid test. 3~6 months later, another blood sample was taken to do the same test as a second round of follow-up test. Results Two rounds follow-up study showed, of the 52 blood donors, 39 donors （75~/oo） turned out to be negative with 4 different anti-HCV ELISA tests, and 13 donors （25~） were re- active results with at least one of the four different anti-HCV ELISA tests, which were the same to the original one, and their S/CO values were basically identical. Of these 13 blood donors with anti-HCV reactive results, 1 blood donor turnsed out to be WB positive. 52 blood donors were negative for the presence of HCV RNA with 2 round Roche cobas Taqscreen MPX test. Conclusion Blood donors who were reactive with 1 of 2 ELISA screening assay, can be reentered after at least 6 months deferral. They were tested to be negative with 2 anti-HCV ELISA assays, inclusive of original assay with reac- tive result, and negative for the presence of HCV RNA.
出处
《临床输血与检验》
CAS
2013年第3期212-216,共5页
Journal of Clinical Transfusion and Laboratory Medicine
