摘要
目的 :比较 4种细胞凋亡检测方法的优缺点。方法 :以 bufalin诱导 HL60细胞凋亡为例 ,选择 4种方法即电镜 ( EM)、DNA电泳、原位缺口标记法 ( TUNEL)和流式细胞术 ( FCM)检测细胞凋亡 ,并对凋亡率进行了比较。结果 :EM和 DNA电泳技术是定性说明凋亡的可靠方法 ,而TUNEL和 FCM擅长于定量检测凋亡细胞 ,EM、TUNEL 和 FCM检测的凋亡率具有显著相关性。结论 :研究细胞凋亡时宜选用既特异、灵敏 。
Objective:To explore the virtues and defects of four different methods for analysis of apoptosis.Methods:Apoptosis of HL60 cells was studied by transmission electron microscopy(EM),agarose gel electrophoresis,TUNEL and flow cytometry(FCM).Results:EM and agarose gel electrophoresis were reliable qualitative methods for analyzing apoptosis,TUNEL and FCM were good at assaying the apoptotic rate quantitatively,the apoptotic rate assayed by EM positively correlated with that by TUNEL and FCM.Conclusion:A specific,sensitve and quantitative method shoule be chosen to detect apoptotic cells.
出处
《武警医学院学报》
CAS
2000年第1期17-20,共4页
Acta Academiae Medicinae CPAPF
关键词
细胞凋亡
方法
比较
Apoptosis
Method
Comparison
