摘要
以西番莲种子为试验材料,在不同的赤霉素浸种时间及不同种子处理方式条件下,进行西番莲种子萌发试验,并利用发芽种子的根进行愈伤组织诱导。结果表明,将西番莲种子剥壳后置于MS培养基中萌发,是西番莲种子萌发的最适方式。根部愈伤组织诱导的最适培养基为MS+0.5 mg/L 2,4-D,诱导出的愈伤组织多为浅绿色,颗粒状,质地疏松。
The germination rate of Passiflora edulis seed and the callus induction of root were studied in different seed soaking time with gibberellin and different seed treatment conditions.The results showed that the shucked Passiflora edulis seed on MS medium was the optimal way for seed germination.The optimal medium of root callus induction was MS＋0.5 mg/L 2,4-D,and the induced callus were mostly light green,granular and loose texture.
出处
《西南农业学报》
CSCD
北大核心
2013年第3期1148-1151,共4页
Southwest China Journal of Agricultural Sciences
基金
国家林业局948项目(2011-4-45)
云南省自然科学基金(2010ZC268)
西南林业大学"大型仪器设备共享基金"
西南林学院生态学重点学科(XKX200902)
关键词
西番莲
种子萌发
愈伤组织
Passiflora edulis
Seed germination
Callus
