摘要
本文以表达性质粒pBD_2为载体,克隆了人乳头瘤病毒第18型(HPV_(18))E_6基因。经DNA分析及蛋白产物分析,筛选出一株重组子(pDV_(11)),可表达分子量与预计相符的新生蛋白质,并绘制了其限制酶酶切图谱。纯化表达蛋白后,经对流免疫电泳鉴定。证明其为预计表达的βga1/E_6融合蛋白。
We have cloned the E6 gene of human papillomavirus type 18 into an expression plasmid pBD2. One of the recombinant plasmids ( namdd pDV11 ) was identified by DNA analysis and protein product analysis. It could express a new protein whose molecular weight corresponded well with the expected one. After purification, the expressed protein showed a positive result in countercurrent immunoelectrophore sis with anti-β-gal serum and was proved to be the expectedβ-gal/E6 fusion protein. The physical map of pDV11 was also prepased.
出处
《第一军医大学学报》
CSCD
1991年第2期100-104,共5页
Journal of First Military Medical University
基金
国家自然科学基金资助项目
关键词
人乳头瘤病毒
重组
表达
基因
克隆
necombinant DNA
human papillomavirus
fusion protein
gene expression
