期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

远端创伤预处理对心肌内向整流钾通道Kir6．2表达的影响 被引量：1

Expression of Kir6. 2 in Rat Myocardial Cells after Remote Preconditioning of Trauma
原文传递
导出
摘要 目的观察远端创伤预处理后心肌细胞内向整流K^＋通道Kit6．2在不同时点的表达变化。方法SD大鼠腹部做一长2cm的横切口后，分别于15min、4h、24h时间点取大鼠左心室心肌，检测其内向整流K^＋通道Kit6．2的蛋白表达的变化。结果远端创伤预处理后，随时间的推移心肌细胞内向整流K^＋通道Kit6．2的蛋白表达量逐渐增加。结论远端创伤预处理可以上调心肌细胞内向整流K^＋通道Kit6．2的表达，这可能参与了远端创伤预处理对心肌缺血再灌注损伤的保护作用。 Objective To investigate the expression of Kit6.2 in myocardial cells after remote preconditioning of trauma （RPCT） at different time points. Methods A transverse incision 2 cm in length was made in a SD rat. Left ventricular myocardial tissue was harvested at 15 rain, 4 h and 24 h after operation. The expression of Kit6. 2 was measured. Results After RPCT, the protein expression level of Kit6.2 in myocardial cells was increased with time. Conclusion RPCT may increase the expression of inward rectifier K＋ channel Kir6. 2 in myocardial cells, suggesting that Kit6.2 may participate in the protection of RPCT in cardiac reperfusion injury （CRI） .
作者 徐林 张登文 张传汉 刘卫 田玉科 王学仁
机构地区 华中科技大学同济医学院附属同济医院麻醉科 济南军区第
出处 《医学分子生物学杂志》 CAS 2013年第3期136-138,共3页 Journal of Medical Molecular Biology
基金 教育部博士点基金新教师项目（No.20070487131）,湖北省卫生厅青年科技人才基金（No.QJX2008-1）
关键词 KATP 心肌保护 缺血再灌注损伤 Kit6.2 myocardial protection ischemia reperfusion injury
分类号 R349.92 [医药卫生—基础医学]
  • 相关文献

参考文献10

  • 1JONES W K, FAN G C, LIAO S, et al. Peripheral nocicep- tion associated with surgical incision elicits remote nonis- chemic cardioprotection via neurogenic activation of pro- tein kinase C signaling [ J ]. Circulation, 2009, 120 ( 11 Suppl) :S1-S9.
  • 2NICHOLS C G. KATP channels as molecular sensors of cellular metabolism [ J ]. Nature, 2006,440 ( 7083 ) : 470- 476.
  • 3POMERANTZ B J, ROBINSON T N, MORRELL T D, et al. Selective mitoehondrial adenosine triphosphate-sensi- tive potassium channel activation is sufficient to precondi- tion human myoeardium [ J ]. J Thorac Cardiovasc Surg, 2000,120 (2) : 387-392.
  • 4HONG M, BAO L, KEFALOYIANNI E, et al. Heterogene- ity of ATP-sensitive K + channels in cardiac myocytes: enrichment at the intercalated disk [ J ]. J Biol Chem, 2012,287 (49) :41258-41267.
  • 5MASLOV L N, LISHMANOV Y B, OELTGEN P R, et al. Activation of peripheral delta2 opioid receptors increases cardiac tolerance to ischemia/reperfusion injury Involve- ment of protein kinase C, NO-synthase, KATP channels and the autonomic nervous system [ J ]. Life Sci, 2009,84 (19-20) :657-663.
  • 6PATEL H H, MOORE J, HSU A K, et al. Cardioprotection at a distance:mesenteric artery occlusion protects the my- ocardium via an opioid sensitive mechanism [ J ]. J Mol Cell Cardiol,2002,34 (10) : 1317-1323.
  • 7CALVERT J W, COETZEE W A, LEFER D J. Novel in- sights into hydrogen sulfide--mediated cytoprotection[ J]. Antioxid Redox Signal,2010,12 (10) : 1203-1217.
  • 8USACHEV Y M,MARSH A J,JOHANNS T M,et al. Ac- tivation of protein kinase C in sensory neurons accelerates Ca2 + uptake into the endoplasmic reticulum [ J ]. J Neu- rosci,2006,26(1) :311-318.
  • 9REN X, WANG Y, JONES W K. TNF-alpha is required for late ischemic preconditioning but not for remote pre- conditioning of trauma [ J ]. J Surg Res, 2004, 121 ( 1 ) : 120-129.
  • 10SEYEDI N,WIN T,LANDER H M,et al. Bradykinin B2- receptor activation augments norepinephrine exocytosis from cardiac sympathetic nerve endings. Mediation by au- tocrine/paracrine mechanisms [ J ] . Circ Res, 1997, 81 (5) :774-784.

同被引文献41

  • 1BACZKO I,HUSTI Z,LANG V,et al. Sarcolemmal KATPchannel modulators and cardiac arrhythmias [ J ]. CurrMed Chem,2011,18(24) :3640-3661.
  • 2KEFALOYIANNI E,BAO L,RINDLER M J,et al. Meas-uring and evaluating the role of ATP-sensitive K + chan-nels in cardiac muscle [ J]. J Mol Cell Cardiol,2012,52(3):596-607.
  • 3MEDEIROS-DOMINGO A,TAN B H’CROTTI L’et al.Gain-of-function mutation S422L in the KCNJ8-encodedcardiac K ( ATP) channel Kii6. 1 as a pathogenic sub-strate for J-wave syndromes [ J ]. Heart Rhythm, 2010, 7(10):1466-1471.
  • 4BAO L’HADJIOLOVA K,COETZEE W A,et al. Endoso-mal KATP channels as a reservoir after myocardial ische-mia :a role for SUR2 subunits [ J ]. Am J Physiol HeartCirc Physiol,2011,300(1) ;H262-H270.
  • 5MANNA P T,SMITH A J,TANEJA T K, et al. Constitu-tive endocytic recycling and protein kinase C-mediated ly-sosomal degradation control K ( ATP) channel surfacedensity[J].J Biol Chem ,2010,285 (8) :5963-5973.
  • 6YAN F,LIN C W,WEISIGER E,et al. Sulfonylureas cor-rect trafficking defects of ATP-sensitive potassium chan-nels caused by mutations in the sulfonylurea receptor[ J].J Biol Chem,2004,279(12) : 11096-11105.
  • 7CHEN P C,BRUEDERLE C E,GAISANO H Y,et al.Syntaxin 1A regulates surface expression of beta-cell ATP-sensitive potassium channels[ J]. Am J Physiol Cell Phys-iol,2011,300(3) :C506-C516.
  • 8ZHOU M,HE H J,SUZUKI R,et al. Localization of sulfo-nylurea receptor subunits, SUR2A and SUR2B, in ratheart[ J]. J Histochem Cytochem,2007 ,55(8) :795-804.
  • 9SMITH A J,SIVAPRASADARAO A. Investigation of K(ATP) channel endocytosis and cell surface density byBiotinylation and Western blotting [ J ]. Methods Mol Biol,2008,491:79-89.
  • 10DHAR-CHOWDHURY P, HARRELL M D,HAN S Y,etal. The glycolytic enzymes, glyceraldehyde-3 -phosphatedehydrogenase,triose-phosphate isomerase,and pyruvatekinase are components of the K( ATP) channel macromo-lecular complex and regulate its function [ J ]. J BiolChem,2005 ,280(46) :38464-38470.

引证文献1

二级引证文献1

医学分子生物学杂志
2013年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部