摘要
Catalytic transfer of genetic information from DNA to RNA is very important in life activities. The unconventional RNA transcription level may be related to the source of genetic diseases. At present, conventional methods for detection of RNA transcripts usually involve cumbersome preparative steps, or require sophisticated laboratory equipments. In this study, we presented a rapid, sensitive nano-detection platform for monitoring of RNA transcript levels. T7 RNA polymerase transcription reaction is employed as the example to test the feasibility of this method. In this design, in vitro synthesized RNA products can be hybridized to the FAM labeled single strand DNA (ssDNA) probes which can be adsorbed onto the graphene oxide (GO) surface. Using GO as the fluorescence switch, excellent capacity of the signal-on fluorescence platform for detection of RNA transcripts level is demonstrated. Transcription levels sensing with this nano-platform achieved a sensitivity of 5 pmol/L transcription template. It is antici- pated that current developed RNA transcript nano-detection mode has the potential to be an alternative to the conventional RNA transcript detection methods.
Catalytic transfer of genetic information from DNA to RNA is very important in life activities. The unconventional RNA tran- scription level may be related to the source of genetic diseases. At present, conventional methods for detection of RNA transcripts usually involve cumbersome preparative steps, or require sophisticated laboratory equipments. In this study, we presented a rapid, sensitive nano-detection platform for monitoring of RNA transcript levels. T7 RNA polymerase transcription reaction is employed as the example to test the feasibility of this method. In this design, in vitro synthesized RNA products can be hybridized to the FAM labeled single strand DNA （ssDNA） probes which can be adsorbed onto the graphene oxide （GO） surface. Using GO as the fluorescence switch, excellent capacity of the signal-on fluorescence platform for detection of RNA transcripts level is demon- strated. Transcription levels sensing with this nano-platform achieved a sensitivity of 5 pmol/L transcription template. It is antici- pated that current developed RNA transcript nano-detection mode has the potential to be an alternative to the conventional RNA transcript detection methods.
基金
supported by the National Basic Research Program of China (2010CB732602)
the Key Program of NSFC-Guangdong Joint Funds of China (U0931005)
the National Natural Science Foundation of China(81101121)
the Natural Science Foundation of Guangdong Province(S2011040005386)
