摘要
作者应用抗人胃癌单克隆抗体MGb_2制备胃癌特异性阿霉素(DXR)免疫脂质体,并观察其特性.电镜负染测得免疫脂质体直径105.6±21.1nm,阿霉素包裹率47.6％±81％,平均每个免疫脂质体包入5667分子DXR,插入48分子MGb_2.ELISA和结合试验证实.免疫脂质体可特异地识别并将其内容物携带至人胃癌靶细胞BGC-823.免疫脂质体对靶细胞显示出选择性杀伤作用,其作用是游离DXR的2.3倍,是非特异性脂质体的56.7倍,但对正常人胚肺细胞SL的毒性只有游离DXR的1.25.结果表明制备的胃癌免疫脂质体对阿霉素具有较好的导向作用,将可能用于胃癌的导向治疗.
Doxombicin Was cntrapped in liposomes coaled with monoclonal antibody MGb2 against human gastric cancer. The liposomes were-prepared by modified reverse-phase evaporation and palmiloyl antibody incorporation method The negative-stain electron microscopy showed that the diameter was 105.6 1 21.1 nm. The drug entrapment efficiency was 47.6% ± 8.1%. There were about 5667 drug molecules entrapped and 48 MGb2 molecules incorporated per one immunoliposome. It was confirmed by FLISA and binding assay that the immunoliposomes could bind selectively and carry their contents to human gastric cancer cell line BGC-823. The immunoliposomes showed specific cytotoxicity to BGC-823, 2 to 3-fold more cffective than that of free DXR and 56.7-fold more effective than that of non-specific liposomes, but only 1.25 of toxicity to normal human embryonic lung cell line SL, as compared with free DXR. These results indicate that the immunoliposomcs may deliver DXR selectively to the target tumor cells and be useful in targeting chemotherapy for human gastric cancer.
出处
《第四军医大学学报》
1991年第1期1-4,共4页
Journal of the Fourth Military Medical University
关键词
胃癌
脂质体
阿霉素
单克隆抗体
liposomes
antibodies, moncdonal
doxorubirin
stomach neoplasms
