摘要
[目的]探究褐飞虱Kruppel homolog-1(Nlkr-h1)基因的锌指结构特点以及对FAMeT和JHE表达的影响,了解Nlkr-h1的调控作用。[方法]通过MEGA5.0分析Nlkr-h1的功能功域每个锌指结构的进化树,并采用qPCR技术测定Nlkr-h1干扰后FAMeT和JHE的表达情况。[结果]Nlkr-h1的功能区域主要包括8个锌指结构,其中Zn1和Zn8的保守性相对较低。在褐飞虱Nlkr-h1基因被敲除的褐飞虱中,发现保幼激素合成酶基因FAMeT和代谢酶基因JHE表达量升高。[结论]为了解保幼激素信号通路分子机理以及褐飞虱的生物防治提供了理论依据。
[Objective]The paper was to explore the characteristics of zinc finger of Kruppel homolog-1（Nlkr-h1） gene from brown planthopper and its effect on expression of FAMeT and JHE,and to understand the regulation role of Nlkr-h1.[Method]The evolutionary tree of each zinc finger in functional area of Nlkr-h1 was analysis via MEGA5.0,and expression status of FAMeT and JHE after interference of Nlkr-h1 was determined using qPCR technology.[Result] The functional area of Nlkr-h1 mainly included eight zinc fingers,and the conservativeness of Zn1 and Zn8 was relatively lower.In the brown planthopper with Nlkr-h1 gene knockout,the expression levels of FAMeT and JHE increased.[Conclusion]The paper provides the theoretical basis for understanding molecular mechanism of juvenile hormone signaling pathway and biological control of brown planthopper.
出处
《安徽农业科学》
CAS
2013年第12期5299-5301,共3页
Journal of Anhui Agricultural Sciences
基金
"973"项目(2010CB126200)
