摘要
本实验以同一来源的约氏疟原虫经血传和蚊传两种方式在小鼠及斯氏按蚊体内传代,观察了其毒力的变化,并对毒力变化的机制进行了探讨。蚊传保种的疟原虫感染小鼠死亡率为13.89％～22.73％,而血传保种疟原虫感染的小鼠10天内全部死亡。长期血传的原虫经一代蚊传感染鼠死亡率降为0％～15％。长期蚊传的原虫连续3代血传感染鼠死亡率达100％。长期血传的疟原虫改嗜成熟红细胞,引起脑部病变。切除脾脏、破坏胸腺不能阻止脑病变的发生。脾脏对鼠疟原虫毒力株无选择作用。
A strain of Plasmodium yoelii was propagated with serial blood passages through mice and with serial transmissions through mosquitoes to investigate the effects of the 2 kinds of propagation on the virulence of the ma-laria parasites. The experiments revealed that the mortality rate of the mice infected with P.yoelii after successive transmissions through mosquitoes was, 13.89% to 22.73%, while those infected with P.yoelii after successive blood passages usually all died within 10 days. When mice were infected with P. yoelii of one generation mosquito transmission, after serial blood passages their mortality rate was 0 % to 15%, when mice were infected with P.yoelii of the third generation blood passages after successive transmissions through mosquitoes, then theirmortalitv rate was 100%.The P.yoelii after serial blood passages mainly invaded the mature erythro-cytes and could cause cerebral symptoms, which could not be prevented by splencctomy or antithymocyte serum therapy. The results of serial passages of the parasite torough splenectomized mice indicate that the spleen can not select the virulent strain of P.yoelii.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1991年第3期265-269,共5页
Journal of Third Military Medical University
关键词
约氏疟原虫
毒力
血传
蚊传
Plasmodium yoelii
virulence
blood passage
mosquito transmission
cerebral ebolism.
