摘要
以多巴胺为模板分子、溴酚蓝为单体,在玻碳电极表面制得分子印迹膜.洗脱后重新吸附多巴胺分子,同时利用聚溴酚蓝膜对多巴胺氧化的电催化作用,对多巴胺进行定量分析.多巴胺在0～1.2×10-6mol/L浓度范围内与电流变化值有线性关系.由于印迹膜的催化作用,检出限达1.62×10-10mol/L.该传感器可用于选择性测定多巴胺,抗坏血酸和尿酸等共存物不干扰测定.利用该传感器直接测定了血清中多巴胺含量,回收率在95.2%～103.0%之间.
A novel strategy for improving the sensitivity of molecular imprinted electrochemical sensor was proposed and dopamine （DA） was selected as the template molecular in this assay. The electroactive membrane of poly-bromophenol blue （BB） which was polymerized on the electrode surface acted as molecular imprinted membrane of DA. To prepare poly-BB-DA molecularly imprinted polymer （MIP）, CV scans were performed for 30 cycles in the potential range between -1.0 and 1.8 V at 50 mV/s in an acetate buffer solution （pH=4.0） containing 1.0×10-3 mol/L DA and 3.0×10-3 mol/L BB at 25 ℃. The MIP electrode was washed by methanol （50% in volume） for 12 min to remove template molecules. Differential pulse voltammetry （DPV） was performed after the rebinding reaction of the DA and the MIP membrane in DA sample solutions for 6 min. With the increasing of the DA concentration, the binding sites in the membrane taken by DA molecules also increased, so was the peak current in the DPV analysis. The sensitivity was improved significantly due to the signal amplifying effect produced by the catalytic effect of electro oxidation of dopamine on BB membrane. The experimental conditions were also optimized. Electrochemical measurements for the MIP membrane characterization were carried out in the supporting electrolyte of 0.01 mol/L K3[Fe（CN）6] solution containing 0.5 mol/L KCl. CV was performed from -0.2 to 0.6 V at a scan rate of 100 mV/s. DPV was performed in the supporting electrolyte of 0.1 mol/L PBS （pH 7.4） over a potential range of -0.1 to 0.6 V, with the pulse amplitude of 50 mV and the scan rate of 50 mV/s. All measurements were carried out at room temperature （25 ℃）. DA was determined by DPV, and there was a linear relationship between oxidation currents and DA concentrations in the range of 0～1.2×10-6 mol/L with a detection limit of 1.62×10-10 mol/L. This is one of the most sensitive sensors for DA determination due to the catalytic oxidation of DA on electroactive MIP.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2013年第6期951-956,共6页
Acta Chimica Sinica
基金
国家自然科学基金(No.21165007)
广西自然科学基金(No.2012GXNSFAA053032)资助~~
关键词
分子印迹
电催化
多巴胺
传感器
聚溴酚蓝
molecular imprint
electro-catalysis
dopamine
sensor
poly-bromophenol blue
