摘要
电离辐射对免疫活性细胞具有明显的生物学效应。小鼠B淋巴细胞杂交瘤细胞受^(60)Coγ线照射1,2.4,6,8 Gy后,细胞活存率、细胞密度、克隆形成率均随照射剂最的增大而直线下降,并具有很好的相关性。克隆形成率的D_(37)值为8.26Gy。抗体分泌总量亦随着照射剂量的增大而直线下降。但每克隆细胞所分泌的抗体量却随着受照剂量的增加而显上升趋势,且具有相关性。结果表明,电离辐射能够刺激抗体分泌细胞分泌抗体的能力,对研究机体辐射损伤时免疫活性细胞的功能变化和探讨增强杂交细胞分泌单克隆抗体的能力具有参考意义。
The mice B lymphocyte hybridoma cells were irradiated by 60Coγ-rays with doses of 1, 2, 4, 6. 8Gy. A markedly dose-relation depression in cell survival rate, cell concentration and clone-forming rate was observed. D37 value of clone-forming rate was 8.26 Gy. Dose-relation depression was also observed in total production of monoclonal antibody of clones. But production of monoclonal antibody per clone was raised with radiation doses. These results indicate that ionization radiation depresses the survival activity of hybridoma cells, but stimulates the secretion of monoclonal antibody of survival cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第6期504-507,共4页
Academic Journal of Second Military Medical University
