摘要
用ELISA以12株单克隆抗体(McAb)对55株E1Tor霍乱弧菌和用遗传学方法突变的28株霍乱弧菌的菌体抗原进行了研究;同时与4种常规分型方法进行对比试验。用12株McAb可将55株E1Tor霍乱弧菌分成11种抗原决定簇和18个McAb型,将遗传学方法的突变株分成9种抗原决定簇和10个McAb型。其中11株McAb 2B_1H_1F_3能识别所有55株E1Tor弧菌和大部分突变株霍乱弧菌,表明有种的特异性;其余4株McAb与各型霍乱弧菌的抗原反应均有差异,对这些菌株的反应百分比各不相同,表明可能存在着亚型或亚群。
In this paper, the antigens of both Vibrio cholerae on 55 strains of El Tor and on 28 strains obtained with genetical method were studied by ELISA method using 12 strains of McAb against Vibrio cholerae (VCOMcAb). The results indicated that the 55 strains of Vibrio cholerae El Tor were expressed 11(A-K) of the recognized antigenic determinants, and fell into XⅧ(Ⅰ-XⅧ) distinct McAb reactivity types or groups, and the 28 varients of Vibrio cholerae from genetical method were expressed 9 (A-Ⅰ) of the recognized antigenic determinants, and fell into Ⅹ (Ⅰ-Ⅹ) distinct McAb reactivity types or groups. From those VCOMcAbs, the “F3” could recognize all 55 strains of Vibrio cholerae El Tor, indicating it has specificity; other 11 strains of VCOMcAb have a variety of reactivity to antigens on the 55 strains of Vibrio cholerae El Tor in both Inaba and Ogawa and their percentages of reactivity are different. This shows that these strains may have subtypes or subgroups.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第6期534-538,共5页
Academic Journal of Second Military Medical University
关键词
单克隆抗体
霍乱弧菌
抗原决定簇
monoclonal antibody
vibrio cholerae
antigenic determinant
