摘要
通过单因素发酵试验和正交试验,确定了枯草芽胞杆菌ACCC-10619最佳培养基配方和发酵条件.结果表明最佳质量分数组合为:麸皮3.0%,葡萄糖3.0%,硫酸铵0.5%,硫酸锰0.05%,发酵温度33℃,初始pH为7.0,发酵周期36 h,静置时间12 h.经100 L发酵罐液体扩大培养,芽胞产量达8.5×1013个/L.为枯草芽胞杆菌微生态制剂生产应用提供参考.
The optimal formula of culture media and fermentation conditions for Bacillus subtilis ACCC-10619 were determined by methods of single factor tests and orthogonal experiments.The results showed that the optimal conditions were as follows： wheat bran 3.0%,glucose 3.0%,ammonium sulfate 0.5%,manganese sulfate 0.05%,initial pH 7.0,cultured at 33 ℃ for 36 h with static time 12 h.The output amounts of spore reached 8.5×1013cfu/L under volume of 100 L expanding fermentation.It could provide reference for production applications of Bacillus subtilis microbial ecological agents.
出处
《晓庄学院自然科学学报》
CAS
北大核心
2013年第3期70-74,共5页
Journal of Natural Science of Hunan Normal University
基金
长沙市科技局留学人员创新基金资助项目(K06801612)
