摘要
采用流式细胞技术对24例卯巢癌患者的新鲜速冻卵巢癌组织的细胞DNA水平及S期比例(S-phase fraction,SPF)进行了定量测定,用亲和酶标法埘新鲜卵巢癌绀织的雌激素受体(Estrogen receptor,ER)、孕激素受体(Progesterone receptor,PR)进行了半定量测定,探讨了卵巢癌类固醇受体表达与DNA水平及SPF之间的关系。结果表明,ER阳性牢与DNA倍体无明显关系,但二倍体组的PR阳性率明显高于异倍体组(P<0.05)。ER+PR+、ER+PR-.ER-PR+的表达在二倍体组与异倍体组无明显差异。受体阴性组平均SPF明显高于ER+PR+组,但与ER+PR-、ER-PR+组无明显差异,SPF<9％、9％～16％和>16％三组间的受体表达也均无明显差异。作者认为,卵巢癌组织ER、PT与细胞DNA水平及S期比例的关系有助于更好地判断预后和选择激素疗法。
Estrogen receptor (ER), progesterone receptor (PR), DNA ploidy level, and S-phase fraction (SPF) of tumor specimens from 24 patients with ovarian carcinoma were analyzed by flow cytometry and affinity enzyme labelling. There was no significant association between ER status and DNA ploidy level, but 75% of diploid tumors were positive for PR as compared with 31% of aneuploid tumors (P<0.05). Receptor-negative tumors had a median S-phase of 18.85% which was significantly higher than the median S-phase of 12.82% in tumors with positive ER and PR (P<0.05). The functional significance of steroid receptor expression in ovarian cancer is unclear, but the association with DNA ploidy level and proliferative activity may allow better identification of prognostic subsets and aid in selecting patients for hormonal therapy.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第2期147-149,共3页
Academic Journal of Second Military Medical University
关键词
卵巢癌
类固醇受体
DNA
S期比例
ovarian carcinoma
steroid receptor
DNA
S-phase fraction
