摘要
利用抗生物素-生物素-过氧化酶复合物技术检查正常鼻粘膜、鼻息肉、鼻内翻性乳头状瘤和鼻乳头状腺癌中的花生凝集素、刀豆凝集素、荆豆凝集体、槐凝集素、麦胚凝集素、豌豆凝集素的受体及癌胚抗原和角蛋白的改变,提示:(1)花生凝集素受体和荆豆凝集素受体在鼻粘膜中的分布与其分化、增生、转化和恶变程度有关:(2)刀豆凝集素受体在鼻粘膜中的分布随其转化和恶变程度增加而增多;(3)同一细胞膜表面可存在有多种凝集索受体:(4)凝集素组织化学检查法较免疫组织化学检查法更具经济、方便、迅速、灵敏的特点。因此,可以利用凝集素来检查鼻部肿瘤的分化、增生、转化和恶变程度,为临床诊断、治疗和预后提供客观指标。
Formalin-fixed paraffin-embedded biopsy specimens of the normal nasal mucosa,nasal polyps, inverted papillomas and papillary adenocarcinomas were analyzed by Avidin Biotin-Peroxidase Complextechnique for the demonstration of lectin receptors including peanut agglutinin (PNA), concanavalin ensifomis agglutinin (ConA),ulex europeaus agglutinin-I (UEA-1), sophora japonica agglutinin (SJA), wheat germ agglutinin (WGA), pisum sativum agglutinin (PSA), carcinoembryonic antigen (CEA) and keratin.lt was found that the distribution of PNA and UEA-I receptors was related to the degree of differentiation, dysplasia or malignant transformation of the nasal mucosa. The quantity of ConA receptors was increased with the degree of transformation of the nasal mucosal cells. There were many kinds of lectin receptors on the surface of one kind of cells. Lectin histochemistry was more economical, convenient, rapid and sensitive than immunohistochemistry. So, lectin histochemistry can be used to examine the degree of differentiation, dysplasia and malignant transformation of nasal neoplastic cells, providing objective index for clinical diagnosis, treatment and prognosis.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第1期54-57,共4页
Academic Journal of Second Military Medical University
关键词
鼻肿瘤
鼻粘膜
凝集素
受体
nasai mucosa
nasal polyp
inverted papilloma
papillary adenocarcinoma
agglutinin
