摘要
目的通过探讨哮喘患儿血清1,25-二羟维生素D3[1,25-dihydroxyvitamin D3,1,25(OH)2D3]与白细胞介素-10浓度(interleukin-10,IL-10)及肺功能的关系,为治疗哮喘患儿提供临床理论依据。方法设36例健康体检儿童为对照组,92例确诊为哮喘的患儿为哮喘组,根据病情程度分为间歇状态(12例)、轻度持续(18例)、中度持续(33例)、重度持续(29例)4个级别;用酶联免疫分析(ELISA)法检测两组血清中1,25(OH)2D3和IL-10的含量;哮喘组肺功能的评价指标为呼气峰流速(PEF)和第1秒用力呼气容积(FEV1)。结果哮喘组血清中1,25(OH)2D3和IL-10的含量均显著低于对照组(均P<0.05);除间歇状态级与轻度持续级患儿在1,25(OH)2D3、IL-10和PEF指标比较差异无统计学意义外,1,25(OH)2D3、IL-10和PEF、FEV1在不同程度的哮喘(轻度持续、中度持续、重度持续)之间两两比较差异均有统计学意义(P<0.05);1,25(OH)2D3与IL-10、PEF和FEV1均呈正相关(P<0.05)。结论哮喘患儿血清1,25(OH)2D3和IL-10水平较健康儿童低,1,25(OH)2D3可以促进IL-10水平提高,改善肺功能。
Objective To explore the correlation of serum 1,25-dihydroxyvitamin D3 [-1,25 （OH）2D3] with level of interleukin-10 （IL-10） and lung function in children with asthma and then to provide clinical theory for Treating. Methods 36 healthy children were chosen as control group,and 92 cases of children with asthma without treatment were considered as asthma group, which were consist of 12 cases of mild intermittent, 18 cases of mild persistent,33 cases of moderate persistent and 29 cases of severe persistent. The levels of 1,25（OH）zD3 and IL-10 in the serum in two groups were measured by enzyme-linked immunosorbent assay （ELISA）. Peak expiratory flow （PEF） and the first second （FEV1） were used as indexes to functional evaluate the lung in asthma group. Results The level of 1,25（OH）2D3 and IL-10 in the Asthma group was significantly lower （P〈0.05） than those in the control group. In serious condition with different degree, except the 1, 25 （OH）2D3, IL-10 and PEF in mild intermittent and mild persistent,there were strong positive relativity （P〈0.05） between 1,25（OH）2D3 and IL-10, 1,25（OH）zD3 and PEF, 1,25 （OH）zD3 and FEVI. Combined with PEF and FEV1 , these four were significantly higher in the higher grade of mild intermittent, mild persistent,moderate persistent and severe persistent （P〈0. 05）. Conclusion The level of serum 1,25（OH）2D3 and IL-10 in healthy children were higher than that in children with asthma,and 1,25（OH）2D3 can up-regulate the level of IL-10 and thus improve the lung function.
出处
《成都医学院学报》
CAS
2013年第3期333-335,共3页
Journal of Chengdu Medical College
