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特异性干扰PB-1基因对H5N1高致病性禽流感病毒复制的影响 被引量：1

Inhibition of Highly Pathogenic Avian Influenza Virus of H5N1 Subtype by the siRNA Specific for PB-1 Gene
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摘要 针对H5N1高致病性禽流感病毒PB-1基因,通过RNA干扰技术,设计siRNA,研究其抑制病毒复制的效果.siRNA转染MDCK细胞并接毒后,病毒滴度测定、Real-time PCR和间接免疫荧光试验结果显示,所设计的siRNA(ps-PB1-777)具有明显的抑制病毒复制的效果,PB-1基因的拷贝数和病毒蛋白表达都下降.设计的siRNA可显著抑制高致病性禽流感病毒的复制.因此,本研究中PB-1基因的有效siRNA可为预防和治疗H5N1高致病性禽流感提供合理的技术支持和物质储备. PBI-specific siRNAs of HSN1 highly pathogenic avian influenza virus （HPAIV） were designed and detected for their abilities to inhibit virus replication in vitro. Two siRNAs （ps-PB1-777 and ps-PB1- 1282） were designed and constructed expression plasmids. Virus titer assay, Real-time PCR and indirect im- muno-fluorescence experiment were used to observe their inhibition effect in MDCK cells challenged with HSN1 HPAIV. The results suggested virus titers of ps-PB1-777 were lower （P〈0.05, 8 times comparing with the control group）; after treatment of ps-PB1-777, the levels of PB-1 mRNA greatly decreased （P〈0.05）; the levels of proteins of HSN1 HPAIV decreased. However, ps-PBl-1282 did not show the abilities to antiviral effect. As a result, ps-PB1-777 can effectively suppressed the replication of HSN1 HPAIV, and the findings might have significant applications for prophylaxis and therapy of influenza virus.
作者 张伟 王铁成 王承宇 杨松涛 高玉伟 夏咸柱
机构地区 大连医科大学微生物学教研室 军事医学科学院军事兽医研究所
出处 《生命科学研究》 CAS CSCD 北大核心 2013年第3期238-242,共5页 Life Science Research
基金 国家科技支撑计划项目(2006BAD06A14)
关键词 高致病性禽流感病毒(HPAIV) 禽流感 干扰RNA PB-1基因 highly pathogenic avian influenza virus（HPAIV） avian influenza virus small interfering RNA PB-1 gene
分类号 R373.13 [医药卫生—病原生物学]
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参考文献12

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二级参考文献18

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生命科学研究
2013年 第3期

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