摘要
目的探讨辣椒碱对结肠癌的转移能力的影响以及辣椒碱产生次效果的生物机制。方法采用MTT法确定辣椒碱对SW 480的最适药物浓度。通过划痕、Transwell、基质胶检测辣椒碱对SW 480的迁移能力的影响。采用免疫印迹法检测EMT、PI3K、STAT3标志蛋白的变化。结果辣椒碱增强了SW 480的迁移和浸润能力,能促进SW 480细胞中VEGF、Vi-mentin、MMP-2、MMP-9的表达。结论辣椒碱对结肠癌的转移有促进作用。
Objective To explore the effect of capsaicin on metastasis ability of colon cancer cells and possible mechanism underlying the effect. Methods MTY assay was used to determine capsaicin optical concentrations, which can affect SW480 cell line. Mobile ability of SW480 was estimated by methods such as scratch test, transwell and matrigel matrix. Expressions of marker proteins were also examined with Western blot assay. Results Capsaicin enhanced the ability of invasion and metastasis of SW480 cells, and increased the expressions of VEGF, Vimentin,MMP-2 and MMP-9 of SW480 cells. Conclusions Capsaicin can promote tumor metastasis of colorectal cancer cells.
出处
《实用医院临床杂志》
2013年第4期39-41,共3页
Practical Journal of Clinical Medicine
关键词
结肠癌
转移
辣椒碱
Colon cancer
Metastasis
Capsaicin
