摘要
以大肠杆菌和乳酸菌穿梭分泌表达型载体pPG612为基本模型,用短乳杆菌S层启动子SlpA替换载体上gusA启动子,并以TGEV N蛋白基因为目基因构建一种乳酸杆菌组成型表达载体,用以检测SlpA启动子在三种不同乳酸杆菌中表达能力。结果表明,该组成型启动子具有表达外源功能蛋白功能,且具有严格宿主选择性,为进一步利用该载体及组成型启动子进行其他应用奠定了基础。
pPG612 is a shuttle vector suitiable for cloning in both E.coli and Lactobacillus. In the current study, we replaced the promoter of pPG612 vector with S-layer protein＇s promoter SIpA of Lactobaccillus brevis, and thus constructed a type of expression vector of lactic acid bacteria, of which the expressing foreign protein ability was detected with the TGEV N gene as a target gene in three lactic acid bacillus. We further confirmed that the vector was able to express TGEV N gene N with SDS-PAGE and western blot. In conclusion, SIpA was a constitutive promoter which could be applied to more studies in the near future.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2013年第6期125-130,共6页
Journal of Northeast Agricultural University
基金
国家863计划项目(2011AA10A213)
关键词
乳酸杆菌
组成型启动子
启动子活性
Lacticacid bacillus
constitutive promoter
promoter activity
