摘要
为探究杨树花不同提取物对小鼠免疫功能影响,将180只雄性小鼠随机分成3组,分别为空白对照组、水提物组和醇提物组,每组60只。灌胃剂量均为0.2 mg.kg-1.d-1,空白对照组灌服灭菌水。15 d后,观察各组小鼠免疫学指标变化。结果表明,水提物能显著提高小鼠免疫器官指数、巨噬细胞吞噬率、巨噬细胞吞噬指数、碳粒廓清吞噬指数、脾淋巴细胞增殖能力、迟发性变态反应水平、血清溶血素抗体积数和抗体生成细胞溶血空斑数;醇提物能显著提高小鼠免疫器官指数、巨噬细胞吞噬率、巨噬细胞吞噬指数、碳粒廓清吞噬指数、血清溶血素抗体积数和抗体生成细胞溶血空斑数,极显著提高脾淋巴细胞增殖能力和迟发性变态反应水平;以上各指标水提物和醇提物组间差异显著。说明杨树花水提物和醇提取物均能增强小鼠免疫功能作用,且醇提物作用更强。
In order to study the effects of poplar flower different extracts on immune function of mice. One hundred and eighty male mice were randomly divided into control group, PWE group and PAE group, 60 mice in each group. All groups adopted intragastric administration by the dose of 0.2 mg. kg-1. d-1. The control group was orally administered sterile water. After 15 d, Changes on immunity function in mice indexes were detected. The results showed that PWE group could improve distinctly immune organs index, phagocytose percentage, phagocytose index, the clearance rate of charcoal particles, proliferation ability, delayed type hypersensitivity reaction, the level of serum hemolysin and number of hemolytic plaque; the PAE group could also enhance remarkably immune organs index, phagocytose percentage, phagocytose index, the clearance rate of charcoal particles, the level of serum hemolysin and number of hemolytic plaque, extremely significantly improve proliferation ability, delayed type hypersensitivity reaction, All immunology indexes of the PAE showed significant differences as compared with the PWE group. These indicated both PWE and the PAE can improve immune function in mice and had effects on improving immune function in mice, and the PAE showed more stronger effects.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2013年第6期86-89,共4页
Journal of Northeast Agricultural University
基金
国家农业科研成果转化资金项目(2012GB000097)
关键词
杨树花提取物
小鼠
免疫功能
Poplar flower extract
mice
immune function
