摘要
聚丙烯酰胺（PAM）是一种油田采油添加剂，在自然奈件下自发降解缓慢，生物降解是PAM无害化处理的新途径。采用平板胁迫法从聚丙烯酰胺生产厂区土壤中分离得到聚丙烯酰胺降解细菌1株，命名为PAMB3。以聚丙烯酰胺为唯一氮源进行生长。通过形态特征、生理生化特征及16SrRNA序列分析，确定其为枯草芽孢杆菌（Bacillussubtilis）。同时分别考查底物浓度、反应温度、初始pH值等条件对PAM降解效果影响。结果表明，PAMB3降解聚丙烯酰胺的适宜条件为pH6～11、温度25～40℃、初始聚丙烯酰胺浓度200～700mg·L-1。在含500mg·L-1聚丙烯酰胺、pH为9的基础培养液中，30℃条件下，培养7d降解率达45．04％。
Polyacrylamide （PAM） is an additive widely used on oil fields. Under natural conditions, spontaneous PAM degradation process is very slow. The bio-degradaUon is a harmless way to deal with PAM. This test adopted a method of tablet stressing preconcentration and separation. A strain of degrading bacteria was screened out from the soil which was taken from chemical plant in which polyacrylamide was produced, Named PAMB3. PAMB3 was able to grow using polyacrylamide as the sole nitrogen sources.With the identification of the morphological characteristics, physiological and biochemical characteristics and 16S rRNA sequence, the PAMB3 was Bacillus substilis. This test researched effect of substrate concentration, reaction temperature and initial pH value on PAM degradation. The results showed that the appropriate conditions of degradation of polyacrylamide were as follows： pH value was from 6.0 to 11.0, temperature was from 25-45 ℃, initial PAM concentration was from 200 to 700 rag. L1. The most effective degradation happened in the PAM concentration 500 mg. L1, pH 9.0 and temperature 30℃. Under this condition, the PAMB3＇s degradation rate was 45.04 % for 7 d.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2013年第5期24-28,共5页
Journal of Northeast Agricultural University
基金
黑龙江省留学回国基金项目(LC04C19)
哈尔滨市科技攻关项目(2010AA4CS024)
关键词
聚丙烯酰胺
生物降解
鉴定
芽孢杆菌
polyacrylamide
biodegradation
identification
Bacillus subtilis
