摘要
目的:研究胰岛素样生长因子ⅡmRNA结合蛋白3(IMP3)在胆管癌中的表达以及其临床意义。方法:免疫组化检测54例胆管癌组织、癌旁组织以及正常组织中IMP3蛋白的表达水平。Western blot检测不同病理分级中IMP3的表达,其中Ⅰ级5例、Ⅱ级4例、Ⅲ级5例。real-time PCR检测17例胆管癌组织、癌旁组织以及正常组织中IMP3 mRNA表达水平。随访统计47例胆管癌术后患者长期生存情况。结果:IMP3在癌组织中呈高表达,阳性率达81.84%,而在癌旁和正常组织中均为阴性。不同病理分级中,IMP3表达量有显著差异,分级越高,IMP3表达量越高。RT-PCR结果显示IMP3的mRNA在癌组织中表达升高,与蛋白表达水平一致。47例术后随访发现IMP3阴性组术后长期生存明显高于IMP3阳性组(P<0.01)。结论:IMP3可以作为胆管癌诊断新的标志物,病理分级越高,恶性程度越高,表达量越高;胆管癌中IMP3高表达提示不良预后。
Objective：To investigate the expression of insulin-like growth factorⅡ mRNA binding 3（IMP3） and its clinical significance.Methods：Paraffin sections of tumor tissues,pericarcinomatous tissues and normal tissues from 54 patients with cholangiocarcinoma were examined for expression of IMP3 by immunohistochemistry.Detect IMP3 expression by Western blot in different pathological grades,including five cases of gradeⅠ,4 cases of gradeⅡ and 5 cases of gradeⅢ.Tumor tissues,pericarcinomatous tissues and normal tissues from 17 patients with cholangiocarcinoma were examined for expression level of IMP3 mRNA by quantitive real time PCR.Follow and count long-term survival of 47 patients with post-operation of cholangiocarcinoma.Results：IMP3 expression was positive in 81.84% of cholangiocarcinoma,negative in pericarcinomatous tissues and normal tissues.Significant differences of IMP3 expression among different pathological grades,the higher grade,the higher IMP3 expression levels.The mRNA level of IMP3 was also increased in tumor tissues,and there was correlation with protein level.The follow up of forty-seven postoperative cases found the postoperative long-term survival of IMP3 negative group was significantly higher than that of the IMP3-positive group（P 0.01）.Conclusion：IMP3 can be used as a new marker for the diagnosis of cholangiocarcinoma,higher pathological grade,the higher degree of malignancy,the higher expression level.The overexpression of IMP3 suggests poor prognosis.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第6期792-796,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助(81273262)
