摘要
目的观察银杏叶提取物(extracts of Ginkgo biloba leaf,EGB)对大鼠脑缺血再灌注氧化损伤的保护作用,为进一步开展复方候选新药筛选研究提供参考依据。方法雄性Wistar大鼠60只,随机等分为①对照组,②模型组,③假手术组,④EGB低剂量组,⑤EGB中剂量组,⑥EGB高剂量组。第①～③组灌胃给予蒸馏水l0 ml/kg,第④～⑥组分别灌胃给予含EGB 25、50、100 mg/kg的蒸馏水混悬液l0 ml/kg,连续14 d。最后一次灌胃后1h,②、④、⑤、⑥组大鼠均结扎双颈总动脉造成不完全性脑缺血1 h,复灌30 min;①组不做处理,③组仅做手术处理不结扎颈总动脉。取各组大鼠大脑皮层制备组织匀浆,检测超氧化物歧化酶(SOD)和一氧化氮合酶(NOS)活性,谷胱甘肽(GSH)和丙二醛(MDA)含量以及总抗氧化能力(T-AOC)等指标的变化。结果与对照组相比,模型组脑组织中的SOD活性、GSH含量和T-AOC显著下降,而NOS活性和MDA含量明显升高;与模型组相比,给于EGB使脑组织中SOD活性、GSH含量和T-AOC明显升高,NOS活性和MDA含量明显降低,中剂量的效果较好。结论 EGB对大鼠脑缺血再灌注氧化损伤具有明显保护作用,且呈一定的剂量相关特性。
Objective We tried to observe the protective effects of extracts of Ginkgo biloba leaf（EGB） on cerebral ischemia/reperfusion induced oxidative injury in rats.Methods Sixty male Wistar rats were randomly divided into following six groups（n=10for each）：（a）normal control,（b） injury model,（c）sham operation,（d）low dose of EGB,（e）middle dose of EGB,（f） high dose of EGB.EGB dissolved in distilled in water at dosages of 25,50 and 100mg/kg was given to group d,e,f by gastric tube,continued for 14d;but group a,b,c was only given the equal volume of distilled water at dose of 10ml/kg.At 1 h after the last drug administration,the rats in group b,d,e,f were submitted to incomplete cerebral ischemia（1h）/reperfusion（30min） injury;but group c was only operated and not submitted to cerebral ischemia/reperfusion injury.The cerebral cortex tissue was taken and homogenized for following index assays：SOD and NOS activities,GSH,MDA content and total antioxidative catacity（T-AOC）.Results Compared with the normal control,the SOD activity,GSH content and T-AOC in rat brain significantly decreased,but NOS activity and MDA content significantly increased in injury model group.Compared with the injury model,SOD activity,GSH content and T-AOC in rat brain significantly increased,but NOS activity and MDA content were significantly decreased after administration of EGB,especially at dosage of 50 mg/kg.Conclusions EGB has protective effects on cerebral ischemia/reperfusion induced oxidative injury in rats and shared the characteristic of dose-related effect.
出处
《空军医学杂志》
2013年第2期93-95,101,共4页
Medical Journal of Air Force
基金
国家重大新药创制科技重大专项课题(2011ZXJ09106-03C)
关键词
银杏叶提取物
脑
自由基
抗氧化功能
Extracts of Ginkgo biloba leaf（EGB）
Brain
Free radical
Antioxidative function
