摘要
目的:通过蒙医三穴组灸疗,观察灸疗对大鼠海马衰老因子的影响。方法:SD大鼠60只,雌雄各半,随机分为正常组、模型组和4个灸疗组,每组10只。对模型组和各灸疗组,连续42d颈背部皮下注射D-半乳糖;正常组连续42d颈背部皮下注射等量的0.9%氯化钠溶液,造模第43天开始三穴组灸疗,1次/隔日,共灸21次。进行水迷宫和水游泳实验并测定胸腺(脾脏)指数、海马匀浆后检测多巴胺(DA)、乙酰胆碱(Ach)、单胺氧化酶(MAO)等含量。结果:与模型组相比,顶会、火衰、肾穴配伍组的DA、MAO、Ach含量无显著性差异,其他配伍组DA和Ach明显提高(P<0.01),MAO活性显著性下降(P<0.01)。除了顶会、火衰、肾穴配伍组外其他组的胸腺、脾脏指数与模型组相比明显提高(P<0.05)。与模型组相比,除顶会、火衰、肾穴配伍组外其他穴位配伍组学习记忆力均显著性提高(P<0.01,P<0.05)。结论:蒙医灸疗能保持机体海马Ach、DA、MAO含量的平衡而达到延缓衰老的作用;也能保持胸腺、脾脏指数,体力能力从而提高免疫力,起到强身保健作用。
Objective： To study the effects on hippocampus aging-factor by Mongolian therapy of moxibustion on three- acupoint. Methods： 60 SD rats, with 30 male and 30 female, were divided into 6 groups including normal group, model group and four treatment groups, with 10 rats in each group. The D-galactose was injected subcutaneously in nap for 42 days for the model and treatment groups, while an equivalent amount of sterile saline for the normal group. Moxibustion on three-acupoint therapy （once every other day, 21 times in total） was conducted for the treatment group. After six weeks of treatment, all rats were tested in water maze and swimming, and then the thymus and spleen indices, and the level of Ach, DA and MAO contents were detected in the hippocampal homogenate. Results： Compared with the model group, DA, MAO and Ach contents in the treatment group consisted Dinghui, Huoshuai and Kidney acupoints showed an insignificant difference, while other acupoints group increased obviously in DA and Ach （P〈0.01）. In contrast, the thymus and spleen indices in other treatment groups significantly increased except Dinghui, Huoshuai and Kidney acupoints groups （P〈0.05）, but an insignificant difference was detected compared with the normal group. Compared with the model group, the treatment group except the dinghui, huoshuai and kidney acupoints showed that the memory and learning abilities were increased obvioushy （P〈0.01, P〈0.05）. Conclusion： Mongolian therapy of moxibustion on three-acupoint could keep the blance of the DA, MAO and Ach contents in hippocampuswhich could defer senility. And it also could keep the thymus, spleen indices and physical strength which could boost immunity and care health.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第7期2016-2019,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
关键词
蒙医灸疗
三穴组穴灸疗
抗衰老
衰老因子
Mongolian moxibustion therapy
Three acupoints group moxibustion therapy
Anti-aging
Aging-factor
