摘要
目的建立高效液相色谱法测定人血浆中吡非尼酮的含量。方法色谱柱为Inspire-C18(150 mm×4.6 mm,5μm),流动相为甲醇-0.2%的磷酸溶液(59∶41,V/V),检测波长为310 nm,流速1 mL.min-1;血浆样品经10%高氯酸溶液沉淀蛋白后直接进样。结果吡非尼酮在0.2～25 mg.L-1范围内呈良好的线性关系(r=1.000)。方法回收率98.7%～106.7%,提取回收率大于73%,日内RSD小于2.6%、日间RSD小于5.9%。结论该方法灵敏,无杂质干扰,分析时间短,简便、准确,可用于临床吡非尼酮血药浓度的定量检测。
AIM To determine pirfenidone in human plasma by HPLC method. METHODS The plasma samples were denatured by 10% perchloric acid and the supernate were analyzed in a Inspire-Cts （150 mm x 4.6 mm, 5 μm） with methanol-0.2% phosphoric acid solution （59 ： 41, V/V） as mobile phase. The flow rate was 1 mL ＂min-1 and detection wavelength was 310 nm. RESULTS The calibration curve of pirfenidone was linear within the range of 0.2 - 25 mg＇L-1, r = 1.000. The method recovery was 98.7% - 106.7% and the extraction recoveries were more than 73%. RSD of inter-day and intra-day was less than 2.6% and 5.9%, respectively. CONCLUSION The method is sensitive with no endogenous interference, simple, accurate and can apply to the determination of clinical therapeutic concentration of pirfenidone.
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
2013年第6期473-476,共4页
Chinese Journal of New Drugs and Clinical Remedies
关键词
吡非尼酮
色谱法
高压液相
血药浓度
药动学
pirfenidone
chromatography, high pressure higuid
plasma concentration
pharmacokinetics
