摘要
研究了微水有机溶剂两相体系中固定化脂肪酶催化的萘普生甲酯的立体选择性水解反应。固定化酶活性受载体极性、水含量、有机溶剂的logP值、产物抑制的影响,据此构建了一种可以连续拆分产生(S)(+)萘普生的微水有机溶剂两相体系。反应在一个具有回路的连续流搅拌反应器中进行,反应器中添加有采用吸附法固定化的脂肪酶,载体为一种弱极性的合成载体,水相连同固定化酶颗粒一起永久保持在反应器中,有机流动相带入底物,带出产物。固定化酶在该50mL反应器中30℃连续操作60d,仅损失活性25%,产生(S)(+)萘普生900mg,产物对映体过量值(eep)为95%。
A stereoselective hydrolysis of the racemic naproxen methyl ester by immobilized lipase from Candida rugosa in a low aqueous organic biphase system was studied. Support polar,water content,the logP value of organic phase and product inhibition effected the activity of immobilized enzyme. According to these reaction conditions, a low aqueous organic biphase system for the continuous production of (S) (+) Naproxen was developed.The reaction was carried out in a continuous flow closed loop 50mL stirred bioreactor packed with YWG C 6H 5, a poorly polar synthetic support on which the lipase had been immobilized by adsorption. The aqueous phase was permanently remained in the reactor associated with the immobilized enzyme particles; the organic phase containing substrate was pumped through this reactor and emerged with the products. The continous flow stirred bioreactor containing 75mg lipase was allowed to operate continuously for 60 days at 30℃ with a 25% loss of activity,900mg of (S) (+) Naproxen (ee p 95%) were producted.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第1期55-59,共5页
Chinese Journal of Biotechnology
基金
中国科学院重点项目
中国科学院西部之光项目
关键词
萘普生
酶法拆分
脂肪酶
微水-有机溶剂两相体
Naproxen, lipase,stereoselective hydrolysis,low aqueous organic biphase system, immobilized enzyme,continuous process
