摘要
目的建立生新发胶囊的质量标准,为其质量控制提供依据。方法采用薄层色谱法对生新发胶囊中的制何首乌、补骨脂、天麻进行定性鉴别,采用高效液相色谱法对生新发胶囊中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷进行含量测定。采用Agilent TC-C18柱(4.6 mm×250 mm,5μm),乙腈-水(15∶85)为流动相,流速1.0 mL/min,柱温为30℃,检测波长320 nm。结果薄层色谱中斑点清晰,2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷0.05～0.50μg范围内呈良好的线性关系,平均回收率为97.8%,RSD=0.39%。结论该方法可行,结果准确,可用于生新发胶囊的质量控制。
Objective To establish the quality standard for Shengxinfa Capsule.Methods TLC was used to identify Praeparata,Psoraleae Fructus and Gastrodiae Rhizoma in Shengxinfa Capsule,and 2,3,5,4’-the four-stilbene-2-O-β-D-glucoside was identified by HPLC.The column of Agilent TC-C18（4.6 mm×250 mm,5 μm） was used.The mobile phase was acetonitrile-water（15∶85）.The flow was 1.0 mL/min and column temperature was 30 ℃.UV detecting wavelength was 320 nm.Results Praeparata,Psoraleae Fructus and Gastrodiae Rhizoma could be identified by TLC.The linear range of 2,3,5,4’-the four-stilbene2-O-β-D-glucoside was in the range of 0.05-0.50 μg.The average recovery was 97.8%（RSD=0.39%）.Conclusion The method is feasible and accurate.The quality of Shengxinfa Capsule can be controlled effectively with the established quality standard.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第7期59-61,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
广西科学研究与技术开发计划(桂科攻1099062-11)
