摘要
原癌基因ｒａｓ是动物中抑制细胞凋亡的重要基因之一。以人的ｒａｓ基因为探针，通过Ｓｏｕｔｈｅｒｎ杂交技术，确证玉米和水稻中均含有ｒａｓ基因的同源序列；同时，运用荧光原位杂交技术，首次对ｒａｓ基因在玉米中的同源序列进行了定位。结果表明：ｒａｓ探针在第２号和第７号染色体上均检出了杂交信号，信号检出率分别为１０．８５％和１４．１５％，杂交信号与着丝粒的百分距离分别为 ５４．９２± １．９０和 ９４．６２± ２．７７。上述研究结果为植物细胞凋亡的进一步研究提供了线索和帮助。
Proto-oncogene ras is an important gene for suppress the aminal cells apoptosis. To analyze the presence of homologous sequences of ras in maize and rice, Southern blot hybridization was performed and positive signals was detected in these two species genomes. The physical location of the ras homologous sequences was also carried out in maize chromosome by FISH. ras was hybridized onto the long arm of the chromosomes 2 and 7. The detection rates of FISH were 10.85% and 14.15%, and Percentage distance from centromere to detection sites were 54.92±1.90 and 94.62± 2.77 respectively. These results provided important clues of the further research of plant apoptosis.
关键词
玉米
细胞凋亡
同源序列
基因定位
原癌基因ras
maize
apoptosis
ras
homologous sequences
fluorescence in situ hybridization
