摘要
:采用蔗糖密度梯度离心法 ( 950 0 0× g,2 h)提取大鼠肝细胞过氧化物酶体 ,所得过氧化物酶体形态完整 ,纯度与肝匀浆相比提高了 2 6倍 ,仅有少量 ( 0 .5%～ 0 .9%)的微粒体和线粒体污染 ,回收率为 1 2 %。为研究过氧化物酶体提供了有效的分离方法。此法还可将过氧化物酶体、微粒体、线粒体同时进行分离。
A modified method for isolating of peroxisomes from rat liver was described. The peroxisome enriched fraction was put onto discontinuous sucrose density gradients and then centrifuged for 2 h at 95000×g. It was found that the peroxisomes obtained with this method were intact, and contained only minute amounts (0.5%～0.9%) cellular mitochondria and microsomes. The purity of the peroxisome was increased 26 fold compared with the liver homogenate. The final yield of peroxisomes was about 12%. The peroxisomes, mitochondria and microsomes could be separated at the same time by this method which provided a basis for the study of peroxisomes.
出处
《氨基酸和生物资源》
CAS
2000年第1期20-22,共3页
Amino Acids & Biotic Resources
